The Complete Chloroplast Genomes of (Orchidaceae) Species: Insight into Genome Structure Divergence and Phylogenetic Analysis

is one of the largest genera and presents some of the most intricate taxonomic problems in the family Orchidaceae, including species of ornamental and medical importance. The lack of knowledge regarding the characterization of chloroplast (cp) genomes has imposed current limitations on our study. Here, we report the complete cp genomes of seven species, including . , . , . , . , . , . , and . , and compared with related taxa to provide a better understanding of their genomic information on taxonomy and phylogeny. A total of 28 cp genomes exhibit typical quadripartite structures with lengths ranging from 145,092 bp to 165,812 bp and a GC content of 36.60% to 38.04%. Each genome contained 125-132 genes, encompassing 74-86 protein-coding genes, 38 tRNA genes, and eight rRNA genes. The genome arrangements, gene contents, and length were similar, with differences observed in gene composition. It is worth noting that there were exogenous fragment insertions in the IR regions of . . A total of 18-49 long repeats and 38-80 simple sequence repeats (SSRs) were detected and the single nucleotide (A/T) was dominant in cp genomes, with an obvious A/T preference. An analysis of relative synonymous codon usage (RSCU) revealed that leucine (Leu) was the most frequently used codon, while cysteine (Cys) was the least used. Six highly variable regions (- > - > - > - > - > -) and five coding sequences ( > > > > ) were identified as potential DNA markers based on nucleotide diversity. Additionally, 31,641 molecular diagnostic characters (MDCs) were identified in complete cp genomes. A phylogenetic analysis based on the complete cp genome sequences and 68 protein-coding genes strongly supported that 28 species can be divided into four branches, sects. , , and , defined by morphology, were non-monophyly. Our results enriched the genetic resources of , providing valuable information to illustrate the complicated taxonomy, phylogeny, and evolution process of the genus.