Rapid Detection of Measles Virus Using Reverse Transcriptase/Recombinase Polymerase Amplification Coupled with CRISPR/Cas12a and a Lateral Flow Detection: A Proof-of-Concept Study

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2024 Mar 13

PMID 38472989

Authors

Elena Pinchon

Steven Henry

Fanny Leon

Chantal Fournier-Wirth

Vincent Foulongne

Jean-Francois Cantaloube

Affiliations

Soon will be listed here.

Abstract

The measles virus is highly contagious, and efforts to simplify its diagnosis are essential. A reverse transcriptase/recombinase polymerase amplification assay coupled with CRISPR/Cas12a and an immunochromatographic lateral flow detection (RT-RPA-CRISPR-LFD) was developed for the simple visual detection of measles virus. The assay was performed in less than 1 h at an optimal temperature of 42 °C. The detection limit of the assay was 31 copies of an RNA standard in the reaction tube. The diagnostic performances were evaluated on a panel of 27 measles virus RT-PCR-positive samples alongside 29 measles virus negative saliva samples. The sensitivity and specificity were 96% (95% CI, 81-99%) and 100% (95% CI, 88-100%), respectively, corresponding to an accuracy of 98% (95% CI, 94-100%; < 0.0001). This method will open new perspectives in the development of the point-of-care testing diagnosis of measles.

Citing Articles

Development and Evaluation of a New Measles Detection Assay Using Real-Time RT-PCR.

Chayeb V, Dolgova A, Popova M, Zheleznova N, Shirobokova S, Shabalina A Int J Mol Sci. 2025; 26(5).

PMID: 40076428 PMC: 11898969. DOI: 10.3390/ijms26051801.

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