Exploring Antioxidative, Cytotoxic and Neuropharmacological Insights into Leaves: Experimental and Approaches

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Mar 12

PMID 38468966

Authors

Kishore Kumar Sarkar

Trina Mitra

Md Aktaruzzaman

Md Ahsan Abid

Md Asibur Rahman

Pradip Debnath

Samir Kumar Sadhu

Affiliations

Soon will be listed here.

Abstract

Background Study: The aim of this research was to examine possible antioxidant, cytotoxic and neurological activity of methanol and n-hexane extracts of leaves. Additionally, we aimed to identify potential lead compounds through analysis.

Methods: antioxidative properties were investigated through different assays, including: total phenolic content assay (TPC), total flavonoid content assay (TFC), DPPH free radical scavenging assay and reducing power assay. Also, the cytotoxic effect of the samples was assessed using the brine shrimp lethality test. In addition, anxiolytic, locomotor, and CNS depressant activities were assessed utilizing various established methods. Moreover, reported compounds were used in the study to explore the best-fit phytoconstituents against gamma-aminobutyric acid (GABA) receptor.

Results: MBOL displayed substantial antioxidative activities in various established assays compared to NBOL. In brine shrimp lethality bioassay, both MBOL and NBOL revealed cytotoxic activity in a concentration-dependent approach. Again, in Elevated Plus Maze test, 200 and 400 mg/kg of NBOL and MBOL demonstrated significant anxiolytic activities evident from time spent in open arms. In addition, maximum number of head dipping was demonstrated by MBOL at 400 mg/kg (53.90 ± 1.16) in Hole Board test. NBOL and MBOL at both doses significantly diminished the magnitude of movements from the 2nd to 5th observation periods in Open Field test. Furthermore, in Hole Cross test, MBOL remarkably dwindled the locomotor activity at 120 min and 180 min (3.60 ± 0.40 and 2.40 ± 0.51) at 400 mg/kg. Finally, analysis revealed 13 compounds as promising leads with strong binding affinity to GABA receptor along with good pharmacokinetics and toxicity profiles.

Conclusion: Therefore, the present study's findings advocate the traditional usage of this plant and recommend both MBOL and NBOL as as a potential source of therapeutic candidate for the management of neurological disorders.

Citing Articles

Comprehensive metabolite profiling and evaluation of anti-nociceptive and anti-inflammatory potencies of (Wurmb.) leaves: Experimental and in-silico approaches.

Islam F, Aktaruzzaman M, Islam M, Rodru F, Yesmine S Heliyon. 2025; 11(3):e42074.

PMID: 39975840 PMC: 11835622. DOI: 10.1016/j.heliyon.2025.e42074.

Identification of acetylcholinesterase inhibitors from traditional medicinal plants for Alzheimer's disease using and machine learning approaches.

Islam M, Aktaruzzaman M, Saif A, Hasan A, Sourov M, Sikdar B RSC Adv. 2024; 14(47):34620-34636.

PMID: 39483377 PMC: 11526779. DOI: 10.1039/d4ra05073h.

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