Synthetic Enhancers Including TFREs Improve Transgene Expression in CHO Cells

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Mar 12

PMID 38468921

Authors

Hui-Ning Liu

Xiao-Yin Wang

Ying Zou

Wen-Bao Wu

Yan Lin

Bo-Yu Ji

Tian-Yun Wang

Affiliations

Soon will be listed here.

Abstract

The human cytomegalovirus major immediate early gene (CMV) promoter is currently the most preferred promoter for recombinant therapeutic proteins (RTPs) production in CHO cells. To enhance the production of RTPs, five synthetic enhancers including multiple transcription factor regulatory elements (TFREs) were evaluated to enhance recombinant protein level in transient and stably transfected CHO cells. Compared with the control, four elements can enhance the report genes expression under both two transfected states. Further, the function of these four enhancers on human serum albumin (HSA) were investigated. We found that the transient expression can increase by up to 1.5 times, and the stably expression can maximum increase by up to 2.14 times. The enhancement of transgene expression was caused by the boost of their corresponding mRNA levels. Transcriptomics analysis was performed and found that transcriptional activation and cell cycle regulation genes were involved. In conclusion, optimization of enhancers in the CMV promoter could increase the production yield of transgene in transfected CHO cells, which has significance for developing high-yield CHO cell expression system.

Citing Articles

From Cell Clones to Recombinant Protein Product Heterogeneity in Chinese Hamster Ovary Cell Systems.

Wang C, Guo X, Wang W, Li J, Wang T Int J Mol Sci. 2025; 26(3).

PMID: 39941092 PMC: 11818180. DOI: 10.3390/ijms26031324.

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