Hard Tissue Formation in Pulpotomized Primary Teeth in Dogs with Nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in Vivo Animal Study

Overview

Journal BMC Oral Health

Publisher Biomed Central

Specialty Dentistry

Date 2024 Mar 12

PMID 38468251

Authors

Sahar Talebi

Nosrat Nourbakhsh

Ardeshir Talebi

Amir Abbas Nourbakhsh

Abbas Haghighat

Maziar Manshayi

Hamid Reza Bakhsheshi

Razieh Karimi

Rahman Nazeri

Kenneth J D MacKenzie

Affiliations

Soon will be listed here.

Abstract

Background: This animal study sought to evaluate two novel nanomaterials for pulpotomy of primary teeth and assess the short-term pulpal response and hard tissue formation in dogs. The results were compared with mineral trioxide aggregate (MTA).

Methods: This in vivo animal study on dogs evaluated 48 primary premolar teeth of 4 mongrel female dogs the age of 6-8 weeks, randomly divided into four groups (n = 12). The teeth underwent complete pulpotomy under general anesthesia. The pulp tissue was capped with MCM-48, MCM-48/Hydroxyapatite (HA), MTA (positive control), and gutta-percha (negative control), and the teeth were restored with intermediate restorative material (IRM) paste and amalgam. After 4-6 weeks, the teeth were extracted and histologically analyzed to assess the pulpal response to the pulpotomy agent.

Results: The data were analyzed using the Kruskal‒Wallis, Fisher's exact, Spearman's, and Mann‒Whitney tests. The four groups were not significantly different regarding the severity of inflammation (P = 0.53), extent of inflammation (P = 0.72), necrosis (P = 0.361), severity of edema (P = 0.52), extent of edema (P = 0.06), or connective tissue formation (P = 0.064). A significant correlation was noted between the severity and extent of inflammation (r = 0.954, P < 0.001). The four groups were significantly different regarding the frequency of bone formation (P = 0.012), extent of connective tissue formation (P = 0.047), severity of congestion (P = 0.02), and extent of congestion (P = 0.01). No bone formation was noted in the gutta-percha group. The type of newly formed bone was not significantly different among the three experimental groups (P = 0.320).

Conclusion: MCM-48 and MCM-48/HA are bioactive nanomaterials that may serve as alternatives for pulpotomy of primary teeth due to their ability to induce hard tissue formation. The MCM-48 and MCM-48/HA mesoporous silica nanomaterials have the potential to induce osteogenesis and tertiary (reparative) dentin formation.

Citing Articles

Titanium- and Strontium-Infused Calcium Silicates Toward Restorative and Regenerative Purposes.

Patel T, S C, Joshy R, Ramasamy P, Ganapathy D Cureus. 2024; 16(5):e60863.

PMID: 38910673 PMC: 11192212. DOI: 10.7759/cureus.60863.

References

Holiel A, Sedek E . Marginal adaptation, physicochemical and rheological properties of treated dentin matrix hydrogel as a novel injectable pulp capping material for dentin regeneration. BMC Oral Health. 2023; 23(1):938. PMC: 10683231. DOI: 10.1186/s12903-023-03677-6. View

Camilleri J . Hydration characteristics of Biodentine and Theracal used as pulp capping materials. Dent Mater. 2014; 30(7):709-15. DOI: 10.1016/j.dental.2014.03.012. View

Mosaddad S, Hussain A, Tebyaniyan H . Exploring the Use of Animal Models in Craniofacial Regenerative Medicine: A Narrative Review. Tissue Eng Part B Rev. 2023; 30(1):29-59. DOI: 10.1089/ten.TEB.2023.0038. View

Dominguez M, Witherspoon D, Gutmann J, Opperman L . Histological and scanning electron microscopy assessment of various vital pulp-therapy materials. J Endod. 2003; 29(5):324-33. DOI: 10.1097/00004770-200305000-00003. View

Sandhyarani B, Noorani H, Shivaprakash P, Dayanand A . Fate of pulpectomized deciduous teeth: Bilateral odontogenic cyst?. Contemp Clin Dent. 2016; 7(2):243-5. PMC: 4906873. DOI: 10.4103/0976-237X.183054. View

Xu Y, You Y, Yi L, Wu X, Zhao Y, Yu J . Dental plaque-inspired versatile nanosystem for caries prevention and tooth restoration. Bioact Mater. 2022; 20:418-433. PMC: 9233191. DOI: 10.1016/j.bioactmat.2022.06.010. View

Koneru B, Shi Y, Wang Y, Chavala S, Miller M, Holbert B . Tetracycline-Containing MCM-41 Mesoporous Silica Nanoparticles for the Treatment of Escherichia coli. Molecules. 2015; 20(11):19690-8. PMC: 6332305. DOI: 10.3390/molecules201119650. View

Nagendrababu V, Kishen A, Murray P, Nekoofar M, de Figueiredo J, Priya E . PRIASE 2021 guidelines for reporting animal studies in Endodontology: a consensus-based development. Int Endod J. 2021; 54(6):848-857. DOI: 10.1111/iej.13477. View

Srinivasan D, Jayanthi M . Comparative evaluation of formocresol and mineral trioxide aggregate as pulpotomy agents in deciduous teeth. Indian J Dent Res. 2011; 22(3):385-90. DOI: 10.4103/0970-9290.87058. View

10.

Zakrzewski W, Dobrzynski M, Zawadzka-Knefel A, Lubojanski A, Dobrzynski W, Janecki M . Nanomaterials Application in Endodontics. Materials (Basel). 2021; 14(18). PMC: 8464804. DOI: 10.3390/ma14185296. View

11.

Pajchel L, Kolodziejski W . Synthesis and characterization of MCM-48/hydroxyapatite composites for drug delivery: Ibuprofen incorporation, location and release studies. Mater Sci Eng C Mater Biol Appl. 2018; 91:734-742. DOI: 10.1016/j.msec.2018.06.028. View

12.

STANLEY H . Criteria for standardizing and increasing credibility of direct pulp capping studies. Am J Dent. 1998; 11 Spec No:S17-34. View

13.

Caicedo R, Abbott P, Alongi D, Alarcon M . Clinical, radiographic and histological analysis of the effects of mineral trioxide aggregate used in direct pulp capping and pulpotomies of primary teeth. Aust Dent J. 2007; 51(4):297-305. DOI: 10.1111/j.1834-7819.2006.tb00447.x. View

14.

Fialho L, Grenho L, Fernandes M, Carvalho S . Porous tantalum oxide with osteoconductive elements and antibacterial core-shell nanoparticles: A new generation of materials for dental implants. Mater Sci Eng C Mater Biol Appl. 2021; 120:111761. DOI: 10.1016/j.msec.2020.111761. View

15.

Celik B, Mutluay M, Arikan V, Sari S . The evaluation of MTA and Biodentine as a pulpotomy materials for carious exposures in primary teeth. Clin Oral Investig. 2018; 23(2):661-666. DOI: 10.1007/s00784-018-2472-4. View

16.

Yoon J, Choi S, Koh J, Lee B, Chang H, Hwang I . Hard tissue formation after direct pulp capping with osteostatin and MTA . Restor Dent Endod. 2021; 46(2):e17. PMC: 8170379. DOI: 10.5395/rde.2021.46.e17. View

17.

Kunert M, Lukomska-Szymanska M . Bio-Inductive Materials in Direct and Indirect Pulp Capping-A Review Article. Materials (Basel). 2020; 13(5). PMC: 7085085. DOI: 10.3390/ma13051204. View

18.

Nosrat A, Peimani A, Asgary S . A preliminary report on histological outcome of pulpotomy with endodontic biomaterials vs calcium hydroxide. Restor Dent Endod. 2013; 38(4):227-33. PMC: 3843034. DOI: 10.5395/rde.2013.38.4.227. View

19.

Guang J, Li J, Hao L . Clinical observation and histopathological evaluation of pulp after pulpotomy of primary teeth with formocresol and biodentine. Cell Mol Biol (Noisy-le-grand). 2022; 68(5):83-88. DOI: 10.14715/cmb/2022.68.5.11. View

20.

Fuks A . Vital pulp therapy with new materials for primary teeth: new directions and treatment perspectives. J Endod. 2008; 34(7 Suppl):S18-24. DOI: 10.1016/j.joen.2008.02.031. View