Studies on Platelet Surface Carbohydrates in Normal and Uraemic Platelets Using 125I-labelled Lectins

Overview

Journal Blut

Specialty Hematology

Date 1985 May 1

PMID 3846457

Citations 1

Authors

M Manso

I de Dios

L Alberca

V Vicente

Affiliations

Soon will be listed here.

Abstract

Binding studies with six different purified 125I-labelled lectins, concanavalin A (con A), wheat germ agglutinin (WGA), Ricinus communis agglutinin II (RCA II), Dolichos biflorus (DB), Tetranolobus purpureus (TP) and P-phyto-hemagglutinin (P-PHA), were used to investigate the surface topography of carbohydrates in platelets from uraemic and normal subjects. Compared with normal the uraemic platelets, bear significantly decreased (more than 2.5-fold) numbers of receptors for P-PHA (N-acetyl D-galactosamine specificity) and Con A (specificity glucose, mannose). The number of WGA, RCA, II, DB and TP receptors in uraemic platelets did not differ from the number in normal platelets. Binding studies with 125I-labelled lectins provide further evidence of molecular defects in uraemic platelets. Moreover, this method might provide a fast and reliable technique for identifying abnormalities in the surface topography of carbohydrates on platelets in several pathological states.

Citing Articles

Detection of anomalies in cell-surface carbohydrates on thrombasthenic platelets using 125I-labeled lectins.

Manso M, de Dios I, Alberca I, Vicente V Blut. 1986; 52(2):91-7.

PMID: 3633202 DOI: 10.1007/BF00321071.

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