Proteomic Analysis of Serum Extracellular Vesicles from Biliary Tract Infection Patients to Identify Novel Biomarkers

Overview

Journal Sci Rep

Specialty Science

Date 2024 Mar 8

PMID 38459197

Authors

Chih-Jung Chang

Yung-Ning Huang

Yang-Bor Lu

Yi Zhang

Ping-Hua Wu

Jian-Shan Huang

Wei Yang

Tung-Ying Chiang

Hui-Shan Hsieh

Wen-Hung Chung

Yu-Chieh Weng

Affiliations

Soon will be listed here.

Abstract

Biliary tract infection (BTI), a commonly occurring abdominal disease, despite being extensively studied for its initiation and underlying mechanisms, continues to pose a challenge in the quest for identifying specific diagnostic biomarkers. Extracellular vesicles (EVs), which emanate from diverse cell types, serve as minute biological entities that mirror unique physiological or pathological conditions. Despite their potential, there has been a relatively restricted exploration of EV-oriented methodologies for diagnosing BTI. To uncover potent protein biomarkers for BTI patients, we applied a label-free quantitative proteomic method known for its unbiased and high-throughput nature. Furthermore, 192 differentially expressed proteins surfaced within EVs isolated from individuals afflicted with BTI. Subsequent GO and KEGG analyses pinpointed Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) and Crumbs homolog 3 (CRB3) as noteworthy biomarkers. Validation via data analysis of plasma-derived EV samples confirmed their specificity to BTI. Our study leveraged an unbiased proteomic tool to unveil CEACAM1 and CRB3 as promising protein biomarkers in serum EVs, presenting potential avenues for the advancement of diagnostic systems for BTI detection.

Citing Articles

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References

Ming G, Guo W, Cheng Y, Wang J . Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis. Transl Pediatr. 2021; 10(8):2083-2094. PMC: 8429877. DOI: 10.21037/tp-21-336. View

Chen C, Shively J . The cell-cell adhesion molecule carcinoembryonic antigen-related cellular adhesion molecule 1 inhibits IL-2 production and proliferation in human T cells by association with Src homology protein-1 and down-regulates IL-2 receptor. J Immunol. 2004; 172(6):3544-52. DOI: 10.4049/jimmunol.172.6.3544. View

Yoshioka Y, Konishi Y, Kosaka N, Katsuda T, Kato T, Ochiya T . Comparative marker analysis of extracellular vesicles in different human cancer types. J Extracell Vesicles. 2013; 2. PMC: 3760642. DOI: 10.3402/jev.v2i0.20424. View

Hu C, Zhang Y, Zhang M, Li T, Zheng X, Guo Q . Exosomal Cripto-1 Serves as a Potential Biomarker for Perihilar Cholangiocarcinoma. Front Oncol. 2021; 11:730615. PMC: 8380828. DOI: 10.3389/fonc.2021.730615. View

Padda J, Khalid K, Khedr A, Patel V, Al-Ewaidat O, Tasnim F . Exosome-Derived microRNA: Efficacy in Cancer. Cureus. 2021; 13(8):e17441. PMC: 8460558. DOI: 10.7759/cureus.17441. View

Simpson R, Lim J, Moritz R, Mathivanan S . Exosomes: proteomic insights and diagnostic potential. Expert Rev Proteomics. 2009; 6(3):267-83. DOI: 10.1586/epr.09.17. View

Lee P, Papachristou G . Management of Severe Acute Pancreatitis. Curr Treat Options Gastroenterol. 2020; 18(4):670-681. PMC: 7673973. DOI: 10.1007/s11938-020-00322-x. View

Huang T, Song C, Zheng L, Xia L, Li Y, Zhou Y . The roles of extracellular vesicles in gastric cancer development, microenvironment, anti-cancer drug resistance, and therapy. Mol Cancer. 2019; 18(1):62. PMC: 6441168. DOI: 10.1186/s12943-019-0967-5. View

Nakajima A, Iijima H, Neurath M, Nagaishi T, Nieuwenhuis E, Raychowdhury R . Activation-induced expression of carcinoembryonic antigen-cell adhesion molecule 1 regulates mouse T lymphocyte function. J Immunol. 2002; 168(3):1028-35. DOI: 10.4049/jimmunol.168.3.1028. View

10.

Liu J, Ren L, Li S, Li W, Zheng X, Yang Y . The biology, function, and applications of exosomes in cancer. Acta Pharm Sin B. 2021; 11(9):2783-2797. PMC: 8463268. DOI: 10.1016/j.apsb.2021.01.001. View

11.

Glaser F, John C, Engel B, Hoh B, Weidemann S, Dieckhoff J . Liver infiltrating T cells regulate bile acid metabolism in experimental cholangitis. J Hepatol. 2019; 71(4):783-792. DOI: 10.1016/j.jhep.2019.05.030. View

12.

Bebelman M, Smit M, Pegtel D, Baglio S . Biogenesis and function of extracellular vesicles in cancer. Pharmacol Ther. 2018; 188:1-11. DOI: 10.1016/j.pharmthera.2018.02.013. View

13.

Zhang Y, Wang Y, Wu W, Liu P, Sun S, Hong M . Elevation of neutrophil carcinoembryonic antigen-related cell adhesion molecule 1 associated with multiple inflammatory mediators was related to different clinical stages in ischemic stroke patients. J Clin Lab Anal. 2022; 36(7):e24526. PMC: 9279952. DOI: 10.1002/jcla.24526. View

14.

Huang Z, Ma L, Huang C, Li Q, Nice E . Proteomic profiling of human plasma for cancer biomarker discovery. Proteomics. 2016; 17(6). DOI: 10.1002/pmic.201600240. View

15.

Margolis L, Sadovsky Y . The biology of extracellular vesicles: The known unknowns. PLoS Biol. 2019; 17(7):e3000363. PMC: 6667152. DOI: 10.1371/journal.pbio.3000363. View

16.

Hanau L, STEIGBIGEL N . Cholangitis: pathogenesis, diagnosis, and treatment. Curr Clin Top Infect Dis. 1995; 15:153-78. View

17.

Coumans F, Brisson A, Buzas E, Dignat-George F, Drees E, El-Andaloussi S . Methodological Guidelines to Study Extracellular Vesicles. Circ Res. 2017; 120(10):1632-1648. DOI: 10.1161/CIRCRESAHA.117.309417. View

18.

Huang D, Sherman B, Lempicki R . Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009; 4(1):44-57. DOI: 10.1038/nprot.2008.211. View

19.

Kiriyama S, Kozaka K, Takada T, Strasberg S, Pitt H, Gabata T . Tokyo Guidelines 2018: diagnostic criteria and severity grading of acute cholangitis (with videos). J Hepatobiliary Pancreat Sci. 2017; 25(1):17-30. DOI: 10.1002/jhbp.512. View

20.

Pietrowska M, Wlosowicz A, Gawin M, Widlak P . MS-Based Proteomic Analysis of Serum and Plasma: Problem of High Abundant Components and Lights and Shadows of Albumin Removal. Adv Exp Med Biol. 2019; 1073:57-76. DOI: 10.1007/978-3-030-12298-0_3. View