Betaine Addition to the Diet Alleviates Intestinal Injury in Growing Rabbits During the Summer Heat Through the AAT/mTOR Pathway

Overview

Journal J Anim Sci Biotechnol

Publisher Biomed Central

Date 2024 Mar 8

PMID 38454493

Authors

Zimei Li

Junning Pu

Xiang Chen

Yanbin Chen

Xiaoyan Peng

Jingyi Cai

Gang Jia

Hua Zhao

Gang Tian

Affiliations

Soon will be listed here.

Abstract

Background: The aim of this experiment was to investigate the effect of different levels of betaine (Bet) inclusion in the diet on the intestinal health of growing rabbits under summer heat. A total of 100 weaned Qixing meat rabbits aged 35 d with body weight of 748.61 ± 38.59 g were randomly divided into 5 treatment groups: control group (basal diet) and Bet groups (basal diet + 0.75, 1.0, 1.5 or 2.0 g/kg Bet). The average daily temperature in the rabbitry during the experiment was 30.48 °C and the relative humidity was 69.44%.

Results: Dietary addition of Bet had no significant effect on growth performance and health status of growing rabbits (P > 0.05), but it increased ileal secretory immunoglobulin A content compared to the control under summer heat (P < 0.05). Addition of 0.75 g/kg Bet up-regulated jejunal IL-4, down-regulated ileal TNF-α expression (P < 0.05). The addition of 1.0 g/kg Bet increased the villi height (VH) in the jejunum (P < 0.05). Serum glucose levels were reduced, and the expression of SLC6A20 was up-regulated in jejunum and ileum of rabbits fed with 1.5 g/kg Bet (P < 0.05). When added at 2.0 g/kg, Bet reduced serum HSP70 content, increased jejunal VH, and up-regulated duodenal SLC7A6, SLC38A2, mTOR and 4EBP-2 expression (P < 0.05). Correlation analysis revealed that intestinal mTOR expression was significantly and positively correlated with SLC7A6, SLC38A2, SLC36A1 and IL-4 expression (P < 0.05).

Conclusions: Dietary addition of Bet can up-regulate the expression of anti-inflammatory factors through the AAT/mTOR pathway, improve the intestinal immune function, alleviate intestinal damage in growing rabbits caused by summer heat, and improve intestinal health.

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References

Quinteiro-Filho W, Ribeiro A, Ferraz-de-Paula V, Pinheiro M, Sakai M, Sa L . Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poult Sci. 2010; 89(9):1905-14. DOI: 10.3382/ps.2010-00812. View

Lian P, Braber S, Garssen J, Wichers H, Folkerts G, Fink-Gremmels J . Beyond Heat Stress: Intestinal Integrity Disruption and Mechanism-Based Intervention Strategies. Nutrients. 2020; 12(3). PMC: 7146479. DOI: 10.3390/nu12030734. View

Akhavan-Salamat H, Ghasemi H . Alleviation of chronic heat stress in broilers by dietary supplementation of betaine and turmeric rhizome powder: dynamics of performance, leukocyte profile, humoral immunity, and antioxidant status. Trop Anim Health Prod. 2015; 48(1):181-8. DOI: 10.1007/s11250-015-0941-1. View

El-Raghi A, Hassan M, Hashem N, Abdelnour S . Struggling Thermal Stress Impacts on Growth Performance and Health Status of Newly Weaned Rabbits Using Nanoemulsion of Considering the Economic Efficiency of Supplementation. Animals (Basel). 2023; 13(11). PMC: 10252083. DOI: 10.3390/ani13111772. View

Ratriyanto A, Mosenthin R . Osmoregulatory function of betaine in alleviating heat stress in poultry. J Anim Physiol Anim Nutr (Berl). 2018; 102(6):1634-1650. DOI: 10.1111/jpn.12990. View

Fu L, Zhang L, Liu L, Yang H, Zhou P, Song F . Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling. Animals (Basel). 2021; 11(11). PMC: 8614368. DOI: 10.3390/ani11113153. View

Kang D, Shim K . Early heat exposure effect on the heat shock proteins in broilers under acute heat stress. Poult Sci. 2021; 100(3):100964. PMC: 7936181. DOI: 10.1016/j.psj.2020.12.061. View

Fritsch S, Weichhart T . Metabolic and immunologic control of intestinal cell function by mTOR. Int Immunol. 2020; 32(7):455-465. DOI: 10.1093/intimm/dxaa015. View

Abdelnour S, Al-Gabri N, Hashem N, Gonzalez-Bulnes A . Supplementation with Proline Improves Haemato-Biochemical and Reproductive Indicators in Male Rabbits Affected by Environmental Heat-Stress. Animals (Basel). 2021; 11(2). PMC: 7913087. DOI: 10.3390/ani11020373. View

10.

El-Ratel I, Tag El-Din T, Bedier M . Beneficial effects of curcumin as a native or nanoparticles form on productive efficiency, liver and kidney functions, antioxidative status and immunity of heat-stressed growing rabbits. J Anim Physiol Anim Nutr (Berl). 2020; 104(6):1778-1787. DOI: 10.1111/jpn.13420. View

11.

Rosario F, Kanai Y, Powell T, Jansson T . Mammalian target of rapamycin signalling modulates amino acid uptake by regulating transporter cell surface abundance in primary human trophoblast cells. J Physiol. 2012; 591(3):609-25. PMC: 3577540. DOI: 10.1113/jphysiol.2012.238014. View

12.

Kiela P, Ghishan F . Physiology of Intestinal Absorption and Secretion. Best Pract Res Clin Gastroenterol. 2016; 30(2):145-59. PMC: 4956471. DOI: 10.1016/j.bpg.2016.02.007. View

13.

Yeo E, Wagner C . Tissue distribution of glycine N-methyltransferase, a major folate-binding protein of liver. Proc Natl Acad Sci U S A. 1994; 91(1):210-4. PMC: 42916. DOI: 10.1073/pnas.91.1.210. View

14.

Alhotan R, Al Sulaiman A, Alharthi A, Abudabos A . Protective influence of betaine on intestinal health by regulating inflammation and improving barrier function in broilers under heat stress. Poult Sci. 2021; 100(9):101337. PMC: 8335656. DOI: 10.1016/j.psj.2021.101337. View

15.

Dos Santos T, Dassi S, Franco C, Costa C, Lee S, Fisher da Silva A . Influence of fibre and betaine on development of the gastrointestinal tract of broilers between hatch and 14 d of age. Anim Nutr. 2019; 5(2):163-173. PMC: 6544582. DOI: 10.1016/j.aninu.2018.06.005. View

16.

Kettunen H, Peuranen S, Tiihonen K . Betaine aids in the osmoregulation of duodenal epithelium of broiler chicks, and affects the movement of water across the small intestinal epithelium in vitro. Comp Biochem Physiol A Mol Integr Physiol. 2001; 129(2-3):595-603. DOI: 10.1016/s1095-6433(01)00298-7. View

17.

Craig S . Betaine in human nutrition. Am J Clin Nutr. 2004; 80(3):539-49. DOI: 10.1093/ajcn/80.3.539. View

18.

Yin L, Li J, Wang H, Yi Z, Wang L, Zhang S . Effects of vitamin B6 on the growth performance, intestinal morphology, and gene expression in weaned piglets that are fed a low-protein diet1. J Anim Sci. 2020; 98(2). PMC: 7023605. DOI: 10.1093/jas/skaa022. View

19.

Dangi S, Dangi S, Chouhan V, Verma M, Kumar P, Singh G . Modulatory effect of betaine on expression dynamics of HSPs during heat stress acclimation in goat (Capra hircus). Gene. 2015; 575(2 Pt 2):543-550. DOI: 10.1016/j.gene.2015.09.031. View

20.

Saghir S, Al Hroob A, Majrashi K, Jaber F, Abduh M, Al-Gabri N . Effects of alginates on the growth, haematological, immunity, antioxidant and pro-inflammatory responses of rabbits under high temperature. Res Vet Sci. 2023; 155:36-43. DOI: 10.1016/j.rvsc.2023.01.002. View