Gene Expression Profile of Mitogen-activated Kinases and MicroRNAs Controlling Their Expression in HaCaT Cell Culture Treated with Lipopolysaccharide A and Cyclosporine A

Overview

Journal Cell Cycle

Specialty Cell Biology

Date 2024 Mar 6

PMID 38445655

Authors

Michal Wojcik

Nikola Zmarzly

Alicja Derkacz

Tomasz Kulpok-Baginski

Natasza Blek

Beniamin Oskar Grabarek

Affiliations

Soon will be listed here.

Abstract

Studies indicate that mitogen-activated protein kinases (MAPKs) are activated and overexpressed in psoriatic lesions. The aim of the study was to assess changes in the expression pattern of genes encoding MAPKs and microRNA (miRNA) molecules potentially regulating their expression in human adult low-calcium high-temperature (HaCaT) keratinocytes exposed to bacterial lipopolysaccharide A (LPS) and cyclosporine A (CsA). HaCaT cells were treated with 1 µg/mL LPS for 8 h, followed by treatment with 100 ng/mL cyclosporine A for 2, 8, or 24 h. Untreated cells served as controls. The molecular analysis consists of microarray, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay analyses. The statistical analysis of the obtained results was performed using Transcriptome Analysis Console and STATISTICA 13.5 PL with the statistical significance threshold of < 0.05. Changes in the expression profile of six mRNAs: dual-specificity phosphatase 1 (, dual-specificity phosphatase 4 (, mitogen-activated protein kinase kinase 2 (, mitogen-activated protein kinase kinase 7 (, mitogen-activated protein kinase kinase kinase 2 ( and mitogen-activated protein kinase 9 ( in cell culture exposed to LPS or LPS and the drug compared to the control. We observed that under the LPS and cyclosporine treatment, the expression o/ miR-34a, miR-1275, miR-3188, and miR-382 changed significantly ( < 0.05). We demonstrated a potential relationship between and miR-34a; and miR-34a, miR-382, and miR-3188; and miR-1275, and mir-200-5p; and mir-200-5p, which may be the subject of further research in the context of psoriasis.

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