Enhancing the Authenticity of Animal By-products: Harmonization of DNA Extraction Methods from Novel Ingredients

Overview

Journal Front Chem

Specialty Chemistry

Date 2024 Mar 6

PMID 38444734

Authors

Andreia Filipa-Silva

Raquel Castro

Mariana Rebelo

Maria J Mota

Andre Almeida

Luisa M P Valente

Sonia Gomes

Affiliations

Soon will be listed here.

Abstract

The increasing global pressure to explore alternative protein sources derived from animal by-products has opened-up opportunities, but it has also created the need to assess their compliance with labelling statements, to ensure consumer's trust in the composition of both feed and food products. Assessing the authenticity of highly processed animal by-products, particularly within the rapidly expanding Halal food market, presents a significant challenge due to the lack of robust and standardized methodologies. However, the success of DNA based authenticity system is highly dependent on the extracted DNA quantity, quality, and purity ratios from heterogeneous matrices. In this work, nine DNA extraction methods were tested on selected processed animal by-products with high-value and interest for the feed industry: meals from poultry meat, blood and feather, and hydrolysates from swine meat and bone, fish, and black soldier fly. The proposed DNA extraction methods are developed to specifically target swine-specific mitochondrial region, as a case study. Both the conventional CTAB method and the commercial kits, specifically Invisorb Spin Tissue Mini and NucleoSpin™ Food, demonstrated superior extraction efficiency and quality ratios. Nevertheless, commercial kits enabled faster detection in comparison to the conventional methods. The absence of swine DNA was successfully validated and confirmed in all animal meals and hydrolysates that did not contain swine in their composition beforehand, demonstrating their compliance with the Halal market requirements.

References

Luo A, Zhang A, Ho S, Xu W, Zhang Y, Shi W . Potential efficacy of mitochondrial genes for animal DNA barcoding: a case study using eutherian mammals. BMC Genomics. 2011; 12:84. PMC: 3042414. DOI: 10.1186/1471-2164-12-84. View

Di Bernardo G, Del Gaudio S, Galderisi U, Cascino A, Cipollaro M . Comparative evaluation of different DNA extraction procedures from food samples. Biotechnol Prog. 2007; 23(2):297-301. DOI: 10.1021/bp060182m. View

Schenk J, Becklund L, Carey S, Fabre P . What is the "modified" CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol. Appl Plant Sci. 2023; 11(3):e11517. PMC: 10278931. DOI: 10.1002/aps3.11517. View

Yin R, Sun Y, Wang K, Feng N, Zhang H, Xiao M . Development of a PCR-based lateral flow strip assay for the simple, rapid, and accurate detection of pork in meat and meat products. Food Chem. 2020; 318:126541. DOI: 10.1016/j.foodchem.2020.126541. View

Premanandh J, Salem S . Progress and challenges associated with halal authentication of consumer packaged goods. J Sci Food Agric. 2017; 97(14):4672-4678. DOI: 10.1002/jsfa.8481. View

Karabasanavar N, Singh S, Kumar D, Shebannavar S . Detection of pork adulteration by highly-specific PCR assay of mitochondrial D-loop. Food Chem. 2013; 145:530-4. DOI: 10.1016/j.foodchem.2013.08.084. View

Faustino M, Veiga M, Sousa P, Costa E, Silva S, Pintado M . Agro-Food Byproducts as a New Source of Natural Food Additives. Molecules. 2019; 24(6). PMC: 6471601. DOI: 10.3390/molecules24061056. View

Demirhan Y, Ulca P, Senyuva H . Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication. Meat Sci. 2011; 90(3):686-9. DOI: 10.1016/j.meatsci.2011.10.014. View

Ali M, Hashim U, Kashif M, Mustafa S, Che Man Y, Abd Hamid S . Development of swine-specific DNA markers for biosensor-based halal authentication. Genet Mol Res. 2012; 11(2):1762-72. DOI: 10.4238/2012.June.29.9. View

10.

Sajali N, Wong S, Hanapi U, Abu Bakar Jamaluddin S, Tasrip N, Desa M . The Challenges of DNA Extraction in Different Assorted Food Matrices: A Review. J Food Sci. 2018; 83(10):2409-2414. DOI: 10.1111/1750-3841.14338. View

11.

Doosti A, Ghasemi Dehkordi P, Rahimi E . Molecular assay to fraud identification of meat products. J Food Sci Technol. 2014; 51(1):148-52. PMC: 3857419. DOI: 10.1007/s13197-011-0456-3. View

12.

Ferreira M, Ribeiro P, Ribeiro L, Barata M, Domingues V, Sousa S . Biofortified Diets Containing Algae and Selenised Yeast: Effects on Growth Performance, Nutrient Utilization, and Tissue Composition of Gilthead Seabream (). Front Physiol. 2022; 12:812884. PMC: 8793800. DOI: 10.3389/fphys.2021.812884. View

13.

Sakalar E, Abasiyanik M, Bektik E, Tayyrov A . Effect of heat processing on DNA quantification of meat species. J Food Sci. 2012; 77(9):N40-4. DOI: 10.1111/j.1750-3841.2012.02853.x. View

14.

Lin P, Huang P, Lee Y, Ng C . Analysis and comparison of protein secondary structures in the rachis of avian flight feathers. PeerJ. 2022; 10:e12919. PMC: 8893027. DOI: 10.7717/peerj.12919. View

15.

Piskata Z, Servusova E, Babak V, Nesvadbova M, Borilova G . The Quality of DNA Isolated from Processed Food and Feed via Different Extraction Procedures. Molecules. 2019; 24(6). PMC: 6471455. DOI: 10.3390/molecules24061188. View

16.

Emons H . Glenn Taylor: The horse who came to dinner: the first criminal case of food fraud. Anal Bioanal Chem. 2019; 411(27):7053-7054. DOI: 10.1007/s00216-019-02107-3. View

17.

Gomes S, Breia R, Carvalho T, Carnide V, Martins-Lopes P . Microsatellite High-Resolution Melting (SSR-HRM) to Track Olive Genotypes: From Field to Olive Oil. J Food Sci. 2018; 83(10):2415-2423. DOI: 10.1111/1750-3841.14333. View

18.

Peano C, Samson M, Palmieri L, Gulli M, Marmiroli N . Qualitative and quantitative evaluation of the genomic DNA extracted from GMO and non-GMO foodstuffs with four different extraction methods. J Agric Food Chem. 2004; 52(23):6962-8. DOI: 10.1021/jf040008i. View

19.

Chalamaiah M, Kumar B, Hemalatha R, Jyothirmayi T . Fish protein hydrolysates: proximate composition, amino acid composition, antioxidant activities and applications: a review. Food Chem. 2012; 135(4):3020-38. DOI: 10.1016/j.foodchem.2012.06.100. View

20.

Montiel-Sosa J, Ruiz-Pesini E, Montoya J, Roncales P, Lopez-Perez M, Perez-Martos A . Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA. J Agric Food Chem. 2000; 48(7):2829-32. DOI: 10.1021/jf9907438. View