The Effects of the Fraction Isolated from Iranian Scorpion Venom on Synaptic Plasticity, Learning, Memory, and Seizure Susceptibility

Overview

Journal Iran J Pharm Res

Publisher Brieflands

Specialty Pharmacology

Date 2024 Mar 6

PMID 38444716

Authors

Elmira Heidarli

Hossein Vatanpour

Nafiseh Nasri Nasrabadi

Maha Soltani

Saeed Tahmasebi

Mehrdad Faizi

Affiliations

Soon will be listed here.

Abstract

Epilepsy, as a neurological disease, can be defined as frequent seizure attacks. Further, it affects many other aspects of patients' mental activities, such as learning and memory. Scorpion venoms have gained notice as compounds with potential antiepileptic properties. Among them, (BS) is one of the Iranian scorpions studied by Aboutorabi et al., who fractionated, characterized, and tested this compound using electrophysiological techniques in brain slices (patch-clamp recording). In the present study, the fraction obtained from gel electrophoresis was investigated through behavioral and electrophysiological assays. At first, ventricular cannulation was performed in rats, and then the active fraction (i.e., F3), carbamazepine, and the vehicle were microinjected into the brain before seizure induction by the subcutaneous (SC) injection of pentylenetetrazol (PTZ). Seizure behaviors were scaled according to Racine stages. Memory and learning were evaluated using the Y-maze and passive avoidance tests. Other groups entered evoked field potential recording after microinjection and seizure induction. Population spike (PS) and field excitatory postsynaptic potential (fEPSP) were measured. The F3 fraction could prevent the fifth stage and postpone the third stage of seizure compared to the control (carbamazepine) group. There was no significant improvement in memory and learning in the group treated with the F3 fraction. Also, PS amplitude and fEPSP slope increased significantly, and long-term potentiation was successfully formed after the high-frequency stimulation of the performant pathway. Our results support the antiepileptic effects of the F3 fraction of BS venom, evidenced by behavioral and electrophysiological studies. However, the effects of this fraction on memory and learning were not in the same direction, suggesting the involvement of two different pathways.

References

Quintero-Hernandez V, Jimenez-Vargas J, Gurrola G, Valdivia H, Possani L . Scorpion venom components that affect ion-channels function. Toxicon. 2013; 76:328-42. PMC: 4089097. DOI: 10.1016/j.toxicon.2013.07.012. View

Herfurth K, Kasper B, Schwarz M, Stefan H, Pauli E . Autobiographical memory in temporal lobe epilepsy: role of hippocampal and temporal lateral structures. Epilepsy Behav. 2010; 19(3):365-71. DOI: 10.1016/j.yebeh.2010.07.012. View

Hu Y, Meng B, Yin S, Yang M, Li Y, Liu N . Scorpion venom peptide HsTx2 suppressed PTZ-induced seizures in mice via the circ_0001293/miR-8114/TGF-β2 axis. J Neuroinflammation. 2022; 19(1):284. PMC: 9713996. DOI: 10.1186/s12974-022-02647-z. View

Novak A, Vizjak K, Rakusa M . Cognitive Impairment in People with Epilepsy. J Clin Med. 2022; 11(1). PMC: 8746065. DOI: 10.3390/jcm11010267. View

Bromer C, Bartol T, Bowden J, Hubbard D, Hanka D, Gonzalez P . Long-term potentiation expands information content of hippocampal dentate gyrus synapses. Proc Natl Acad Sci U S A. 2018; 115(10):E2410-E2418. PMC: 5877922. DOI: 10.1073/pnas.1716189115. View

Nassiri-Asl M, Zamansoltani F, Javadi A, Ganjvar M . The effects of rutin on a passive avoidance test in rats. Prog Neuropsychopharmacol Biol Psychiatry. 2009; 34(1):204-7. DOI: 10.1016/j.pnpbp.2009.11.006. View

Zhao R, Zhang X, Yang J, Weng C, Jiang L, Zhang J . Anticonvulsant effect of BmK IT2, a sodium channel-specific neurotoxin, in rat models of epilepsy. Br J Pharmacol. 2008; 154(5):1116-24. PMC: 2451059. DOI: 10.1038/bjp.2008.156. View

Wahab A . Difficulties in Treatment and Management of Epilepsy and Challenges in New Drug Development. Pharmaceuticals (Basel). 2016; 3(7):2090-2110. PMC: 4036655. DOI: 10.3390/ph3072090. View

Migaud M, Charlesworth P, Dempster M, Webster L, Watabe A, Makhinson M . Enhanced long-term potentiation and impaired learning in mice with mutant postsynaptic density-95 protein. Nature. 1998; 396(6710):433-9. DOI: 10.1038/24790. View

10.

Possani L, Becerril B, Delepierre M, Tytgat J . Scorpion toxins specific for Na+-channels. Eur J Biochem. 1999; 264(2):287-300. DOI: 10.1046/j.1432-1327.1999.00625.x. View

11.

Han T, Qin Y, Mou C, Wang M, Jiang M, Liu B . Seizure induced synaptic plasticity alteration in hippocampus is mediated by IL-1β receptor through PI3K/Akt pathway. Am J Transl Res. 2016; 8(10):4499-4509. PMC: 5095344. View

12.

Rodriguez de la Vega R, Possani L . Current views on scorpion toxins specific for K+-channels. Toxicon. 2004; 43(8):865-75. DOI: 10.1016/j.toxicon.2004.03.022. View

13.

Wang X, Zhu D, Li N, Huang Y, Wang Y, Zhang T . Scorpion Venom Heat-Resistant Peptide is Neuroprotective against Cerebral Ischemia-Reperfusion Injury in Association with the NMDA-MAPK Pathway. Neurosci Bull. 2019; 36(3):243-253. PMC: 7056763. DOI: 10.1007/s12264-019-00425-1. View

14.

Aboutorabi A, Naderi N, Pourbadie H, Zolfagharian H, Vatanpour H . The Effects of Scorpion Venom on Electrophysiological Properties of Magnocellular Neurons of Rat Supraoptic Nucleus. Iran J Pharm Res. 2018; 17(1):184-192. PMC: 5937089. View

15.

Das R, Luczak A . Epileptic seizures and link to memory processes. AIMS Neurosci. 2022; 9(1):114-127. PMC: 8941196. DOI: 10.3934/Neuroscience.2022007. View

16.

Cooke S, Bliss T . Plasticity in the human central nervous system. Brain. 2006; 129(Pt 7):1659-73. DOI: 10.1093/brain/awl082. View

17.

Rayner G, Jackson G, Wilson S . Mechanisms of memory impairment in epilepsy depend on age at disease onset. Neurology. 2016; 87(16):1642-1649. PMC: 5085077. DOI: 10.1212/WNL.0000000000003231. View

18.

Franzini-Armstrong C, Protasi F . Ryanodine receptors of striated muscles: a complex channel capable of multiple interactions. Physiol Rev. 1997; 77(3):699-729. DOI: 10.1152/physrev.1997.77.3.699. View

19.

Bruel-Jungerman E, Davis S, Rampon C, Laroche S . Long-term potentiation enhances neurogenesis in the adult dentate gyrus. J Neurosci. 2006; 26(22):5888-93. PMC: 6675234. DOI: 10.1523/JNEUROSCI.0782-06.2006. View

20.

Tahmasebi S, Oryan S, Mohajerani H, Akbari N, Palizvan M . Probiotics and Nigella sativa extract supplementation improved behavioral and electrophysiological effects of PTZ-induced chemical kindling in rats. Epilepsy Behav. 2020; 104(Pt A):106897. DOI: 10.1016/j.yebeh.2019.106897. View