In Vitro Immunoregulatory Role of Recombinant Ancylostoma Ceylanicum Calreticulin

Overview

Journal Parasites Hosts Dis

Specialties Parasitology
Tropical Medicine

Date 2024 Mar 5

PMID 38443772

Authors

Tingting Zhuang

Asmaa M I Abuzeid

Xiaoyu Chen

Shilan Zhu

Guoqing Li

Affiliations

Soon will be listed here.

Abstract

Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes the intestines of humans and animals (dogs and cats), leading to malnutrition and iron-deficiency anemia. Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host's immune response. However, no data on A. ceylanicum calreticulin (Ace-CRT) are available. We investigated the biological function of recombinant Ace-CRT (rAce-CRT). rAce-CRT showed reliable antigenicity and stimulated the proliferation of mouse splenocytes and canine peripheral blood mononuclear cells. Quantitative reverse-transcription PCR assays revealed that rAce-CRT primarily promoted the expression of T helper 2 cytokines, particularly IL-13, in canine peripheral blood lymphocytes. rAce-CRT inhibited complement-mediated sheep erythrocyte hemolysis in vitro. Our findings indicate that Ace-CRT plays an immunomodulatory role and may be a promising candidate molecule for a hookworm vaccine.

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