Molecular Identification of in Tissues of Wild Boars () in the Border Region Between Brazil and Uruguay

Overview

Journal J Parasit Dis

Specialty Parasitology

Date 2024 Mar 5

PMID 38440759

Authors

Gilneia da Rosa

Isac Junior Roman

Leticia Trevisan Gressler

Juliana Felipetto Cargnelutti

Fernanda Silveira Flores Vogel

Affiliations

Soon will be listed here.

Abstract

, owing to its clinical importance in domestic animals, is currently one of the most studied agents, presenting a wide range of intermediate hosts that have not yet been described, mainly in wild fauna. Thus, the aim of this study was to describe the detection and molecular detection of by amplification of the 18S rRNA region in the tissues of wild boars killed by boar control program in border Brazil Uruguay. A total of 79 samples of DNA from wild boar tissues from the LADOPAR/UFSM sampling bank were used, with Nested-PCR reactions being performed for amplification of the 18S rRNA region and the expected final product of 290 bp. Subsequently, the positive samples were subjected to restriction fragment length polymorphism (RFLP) technique with the restriction enzymes and . A second semi-Nested reaction was performed to obtain a larger sequence of nucleotides with amplification of the 18S region and the expected final product of 500 bp for and Nested amplification ITS1 with product final of 367 pb. In 32 samples, it was possible to detect both by nested Nested-PCR reaction and RFLP, and the presence of the agent was confirmed by sequencing, corresponding to 40.51% of the total tissues evaluated. This is the first report of the occurrence of this species of in wild boars, and further studies evaluating the role of these animals as intermediate hosts, and in the epidemiology of this protozoan are necessary, as well as verifying the risk factors for infection.

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