Evaluating the Performance of OCT in Assessing Static and Potential Dynamic Properties of the Retinal Ganglion Cells and Nerve Fiber Bundles in the Living Mouse Eye

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2024 Feb 29

PMID 38420317

Authors

Pengfei Zhang

Olga Vafaeva

Christian Dolf

Yanhong Ma

Guozhen Wang

Jessicca Cho

Henry Ho-Lung Chan

Nicholas Marsh-Armstrong

Robert J Zawadzki

Affiliations

Soon will be listed here.

Abstract

Glaucoma is a group of eye diseases characterized by the thinning of the retinal nerve fiber layer (RNFL), which is primarily caused by the progressive death of retinal ganglion cells (RGCs). Precise monitoring of these changes at a cellular resolution in living eyes is significant for glaucoma research. In this study, we aimed to assess the effectiveness of temporal speckle averaging optical coherence tomography (TSA-OCT) and dynamic OCT (dOCT) in examining the static and potential dynamic properties of RGCs and RNFL in living mouse eyes. We evaluated parameters such as RNFL thickness and possible dynamics, as well as compared the ganglion cell layer (GCL) soma density obtained from OCT, fluorescence scanning laser ophthalmoscopy (SLO), and histology.

Citing Articles

Line-field dynamic optical coherence tomography platform for volumetric assessment of biological tissues.

Chen K, Swanson S, Bizheva K Biomed Opt Express. 2024; 15(7):4162-4175.

PMID: 39022542 PMC: 11249681. DOI: 10.1364/BOE.527797.

References

Grieve K, Thouvenin O, Sengupta A, Borderie V, Paques M . Appearance of the Retina With Full-Field Optical Coherence Tomography. Invest Ophthalmol Vis Sci. 2016; 57(9):OCT96-OCT104. DOI: 10.1167/iovs.15-18856. View

Jonas J, Aung T, Bourne R, Bron A, Ritch R, Panda-Jonas S . Glaucoma. Lancet. 2017; 390(10108):2183-2193. DOI: 10.1016/S0140-6736(17)31469-1. View

Erchova I, Tumlinson A, Fergusson J, White N, Drexler W, Sengpiel F . Optophysiological Characterisation of Inner Retina Responses with High-Resolution Optical Coherence Tomography. Sci Rep. 2018; 8(1):1813. PMC: 5788978. DOI: 10.1038/s41598-018-19975-x. View

Hedberg-Buenz A, Christopher M, Lewis C, Fernandes K, Dutca L, Wang K . Quantitative measurement of retinal ganglion cell populations via histology-based random forest classification. Exp Eye Res. 2015; 146:370-385. PMC: 4841761. DOI: 10.1016/j.exer.2015.09.011. View

Miller D, Grannonico M, Liu M, Kuranov R, Netland P, Liu X . Visible-Light Optical Coherence Tomography Fibergraphy for Quantitative Imaging of Retinal Ganglion Cell Axon Bundles. Transl Vis Sci Technol. 2020; 9(11):11. PMC: 7552935. DOI: 10.1167/tvst.9.11.11. View

Schmidt E, Kus L, Gong S, Heintz N . BAC transgenic mice and the GENSAT database of engineered mouse strains. Cold Spring Harb Protoc. 2013; 2013(3). DOI: 10.1101/pdb.top073692. View

Yu D, Cringle S, Balaratnasingam C, Morgan W, Yu P, Su E . Retinal ganglion cells: Energetics, compartmentation, axonal transport, cytoskeletons and vulnerability. Prog Retin Eye Res. 2013; 36:217-46. DOI: 10.1016/j.preteyeres.2013.07.001. View

Zhang P, Zam A, Jian Y, Wang X, Li Y, Lam K . In vivo wide-field multispectral scanning laser ophthalmoscopy-optical coherence tomography mouse retinal imager: longitudinal imaging of ganglion cells, microglia, and Müller glia, and mapping of the mouse retinal and choroidal vasculature. J Biomed Opt. 2015; 20(12):126005. PMC: 4681314. DOI: 10.1117/1.JBO.20.12.126005. View

Leung C, Weinreb R, Wei Li Z, Liu S, Lindsey J, Choi N . Long-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells. Invest Ophthalmol Vis Sci. 2011; 52(3):1539-47. DOI: 10.1167/iovs.10-6012. View

10.

Kurokawa K, Crowell J, Zhang F, Miller D . Suite of methods for assessing inner retinal temporal dynamics across spatial and temporal scales in the living human eye. Neurophotonics. 2020; 7(1):015013. PMC: 7070771. DOI: 10.1117/1.NPh.7.1.015013. View

11.

Yadav K, Rajpurohit R, Sharma S . Glaucoma: Current treatment and impact of advanced drug delivery systems. Life Sci. 2019; 221:362-376. DOI: 10.1016/j.lfs.2019.02.029. View

12.

Hillmann D, Spahr H, Pfaffle C, Sudkamp H, Franke G, Huttmann G . In vivo optical imaging of physiological responses to photostimulation in human photoreceptors. Proc Natl Acad Sci U S A. 2016; 113(46):13138-13143. PMC: 5135337. DOI: 10.1073/pnas.1606428113. View

13.

Daniel S, Clark A, McDowell C . Subtype-specific response of retinal ganglion cells to optic nerve crush. Cell Death Discov. 2018; 4:7. PMC: 6054657. DOI: 10.1038/s41420-018-0069-y. View

14.

Kuehn M, Fingert J, Kwon Y . Retinal ganglion cell death in glaucoma: mechanisms and neuroprotective strategies. Ophthalmol Clin North Am. 2005; 18(3):383-95, vi. DOI: 10.1016/j.ohc.2005.04.002. View

15.

Scholler J, Mazlin V, Thouvenin O, Groux K, Xiao P, Sahel J . Probing dynamic processes in the eye at multiple spatial and temporal scales with multimodal full field OCT. Biomed Opt Express. 2019; 10(2):731-746. PMC: 6377896. DOI: 10.1364/BOE.10.000731. View

16.

Zhang L, Dong R, Zawadzki R, Zhang P . Volumetric data analysis enabled spatially resolved optoretinogram to measure the functional signals in the living retina. J Biophotonics. 2021; 15(3):e202100252. PMC: 8901551. DOI: 10.1002/jbio.202100252. View

17.

Soto I, Oglesby E, Buckingham B, Son J, Roberson E, Steele M . Retinal ganglion cells downregulate gene expression and lose their axons within the optic nerve head in a mouse glaucoma model. J Neurosci. 2008; 28(2):548-61. PMC: 6670511. DOI: 10.1523/JNEUROSCI.3714-07.2008. View

18.

McKinnon S, Schlamp C, Nickells R . Mouse models of retinal ganglion cell death and glaucoma. Exp Eye Res. 2008; 88(4):816-24. PMC: 3056071. DOI: 10.1016/j.exer.2008.12.002. View

19.

Munter M, Vom Endt M, Pieper M, Casper M, Ahrens M, Kohlfaerber T . Dynamic contrast in scanning microscopic OCT. Opt Lett. 2020; 45(17):4766-4769. DOI: 10.1364/OL.396134. View

20.

Kohlfaerber T, Pieper M, Munter M, Holzhausen C, Ahrens M, Idel C . Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways. Biomed Opt Express. 2022; 13(6):3211-3223. PMC: 9208592. DOI: 10.1364/BOE.456104. View