A Prototrophic Suppressor of a D-glutamate Auxotroph Reveals a Member of the Periplasmic Broad-spectrum Racemase Family (BsrF)

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2024 Feb 27

PMID 38411059

Authors

Macey N Coppinger

Kathrin Laramore

David L Popham

Eric V Stabb

Affiliations

Soon will be listed here.

Abstract

Importance: D-Amino acids are used and produced by organisms across all domains of life, but often, their origins and roles are not well understood. In bacteria, D-ala and D-glu are structural components of the canonical peptidoglycan cell wall and are generated by dedicated racemases Alr and MurI, respectively. The more recent discovery of additional bacterial racemases is broadening our view and deepening our understanding of D-amino acid metabolism. Here, while exploring alternative PG biosynthetic pathways in , we unexpectedly shed light on an unusual racemase, BsrF. Our results illustrate a novel mechanism for the evolution of antibiotic resistance and provide a new avenue for exploring the roles of non-canonical racemases and D-amino acids in bacteria.

Citing Articles

Transient infection of with an engineered D-alanine auxotroph of .

Coppinger M, Yang L, Popham D, Ruby E, Stabb E Appl Environ Microbiol. 2024; 90(10):e0129824.

PMID: 39235243 PMC: 11497789. DOI: 10.1128/aem.01298-24.

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