Regulated Production of an Influenza Virus Spliced MRNA Mediated by Virus-specific Products

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 Sep 1

PMID 3841059

Citations 17

Authors

D B Smith

S C Inglis

Affiliations

Soon will be listed here.

Abstract

The influenza virus NS2 mRNA is generated through processing by cellular enzymes of a transcript (the NS1 mRNA) of virion RNA segment 8. Production of this mRNA is altered in cells infected with a mutant of influenza A (fowl plague) virus. The proportion of segment 8 transcripts which accumulated in a spliced form was found to be considerably lower in mutant virus-infected cells than in cells infected with wild-type virus, and the amplification in production of NS2 mRNA relative to that of the NS1 mRNA, which normally occurs during infection with wild-type virus, was not observed with the mutant. The NS1 mRNA specified by the mutant virus has unaltered splice recognition sites and was apparently processed normally during a mixed infection with a strain of virus which is wild-type for production of NS2 mRNA. These results suggest that the production of NS2 mRNA is regulated by virus-specific products; these products may act by increasing the efficiency of splicing of NS1 mRNA.

Citing Articles

Dissecting the mechanism of signaling-triggered nuclear export of newly synthesized influenza virus ribonucleoprotein complexes.

Schreiber A, Boff L, Anhlan D, Krischuns T, Brunotte L, Schuberth C Proc Natl Acad Sci U S A. 2020; 117(28):16557-16566.

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Influenza viruses and mRNA splicing: doing more with less.

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Biogenesis, assembly, and export of viral messenger ribonucleoproteins in the influenza A virus infected cell.

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Mutation analysis of a recombinant NS replicon shows that influenza virus NS1 protein blocks the splicing and nucleo-cytoplasmic transport of its own viral mRNA.

Garaigorta U, Ortin J Nucleic Acids Res. 2007; 35(14):4573-82.

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Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway.

Elton D, Simpson-Holley M, Archer K, Medcalf L, Hallam R, McCauley J J Virol. 2000; 75(1):408-19.

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