Characterization of Sucrose Nonfermenting-1-related Protein Kinase 2 (SnRK2) Gene Family in Haynaldia Villosa Demonstrated SnRK2.9-V Enhances Drought and Salt Stress Tolerance of Common Wheat

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2024 Feb 26

PMID 38408894

Authors

Jia Liu

Luyang Wei

Yirong Wu

Zongkuan Wang

Haiyan Wang

Jin Xiao

Xiue Wang

Li Sun

Affiliations

Soon will be listed here.

Abstract

Background: The sucrose nonfermenting-1-related protein kinase 2 (SnRK2) plays a crucial role in responses to diverse biotic/abiotic stresses. Currently, there are reports on these genes in Haynaldia villosa, a diploid wild relative of wheat.

Results: To understand the evolution of SnRK2-V family genes and their roles in various stress conditions, we performed genome-wide identification of the SnRK2-V gene family in H. villosa. Ten SnRK2-V genes were identified and characterized for their structures, functions and spatial expressions. Analysis of gene exon/intron structure further revealed the presence of evolutionary paths and replication events of SnRK2-V gene family in the H. villosa. In addition, the features of gene structure, the chromosomal location, subcellular localization of the gene family were investigated and the phylogenetic relationship were determined using computational approaches. Analysis of cis-regulatory elements of SnRK2-V gene members revealed their close correlation with different phytohormone signals. The expression profiling revealed that ten SnRK2-V genes expressed at least one tissue (leave, stem, root, or grain), or in response to at least one of the biotic (stripe rust or powdery mildew) or abiotic (drought or salt) stresses. Moreover, SnRK2.9-V was up-regulated in H. villosa under the drought and salt stress and overexpressing of SnRK2.9-V in wheat enhanced drought and salt tolerances via enhancing the genes expression of antioxidant enzymes, revealing a potential value of SnRK2.9-V in wheat improvement for salt tolerance.

Conclusion: Our present study provides a basic genome-wide overview of SnRK2-V genes in H. villosa and demonstrates the potential use of SnRK2.9-V in enhancing the drought and salt tolerances in common wheat.

References

Xing L, Hu P, Liu J, Witek K, Zhou S, Xu J . Pm21 from Haynaldia villosa Encodes a CC-NBS-LRR Protein Conferring Powdery Mildew Resistance in Wheat. Mol Plant. 2018; 11(6):874-878. DOI: 10.1016/j.molp.2018.02.013. View

McLoughlin F, Galvan-Ampudia C, Julkowska M, Caarls L, Van der Does D, Lauriere C . The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress. Plant J. 2012; 72(3):436-49. PMC: 3533798. DOI: 10.1111/j.1365-313X.2012.05089.x. View

Tian S, Mao X, Zhang H, Chen S, Zhai C, Yang S . Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat. J Exp Bot. 2013; 64(7):2063-80. PMC: 3638835. DOI: 10.1093/jxb/ert072. View

Umezawa T, Yoshida R, Maruyama K, Yamaguchi-Shinozaki K, Shinozaki K . SRK2C, a SNF1-related protein kinase 2, improves drought tolerance by controlling stress-responsive gene expression in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2004; 101(49):17306-11. PMC: 535404. DOI: 10.1073/pnas.0407758101. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Lefort V, Longueville J, Gascuel O . SMS: Smart Model Selection in PhyML. Mol Biol Evol. 2017; 34(9):2422-2424. PMC: 5850602. DOI: 10.1093/molbev/msx149. View

Shao Y, Qin Y, Zou Y, Ma F . Genome-wide identification and expression profiling of the SnRK2 gene family in Malus prunifolia. Gene. 2014; 552(1):87-97. DOI: 10.1016/j.gene.2014.09.017. View

Ying S, Zhang D, Li H, Liu Y, Shi Y, Song Y . Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis. Plant Cell Rep. 2011; 30(9):1683-99. DOI: 10.1007/s00299-011-1077-z. View

Hernandez-Garcia C, Finer J . Identification and validation of promoters and cis-acting regulatory elements. Plant Sci. 2014; 217-218:109-19. DOI: 10.1016/j.plantsci.2013.12.007. View

10.

Shewry P . Wheat. J Exp Bot. 2009; 60(6):1537-53. DOI: 10.1093/jxb/erp058. View

11.

Zhao G, Zou C, Li K, Wang K, Li T, Gao L . The Aegilops tauschii genome reveals multiple impacts of transposons. Nat Plants. 2017; 3(12):946-955. DOI: 10.1038/s41477-017-0067-8. View

12.

Tan W, Zhang D, Zhou H, Zheng T, Yin Y, Lin H . Transcription factor HAT1 is a substrate of SnRK2.3 kinase and negatively regulates ABA synthesis and signaling in Arabidopsis responding to drought. PLoS Genet. 2018; 14(4):e1007336. PMC: 5919683. DOI: 10.1371/journal.pgen.1007336. View

13.

Szymanska K, Polkowska-Kowalczyk L, Lichocka M, Maszkowska J, Dobrowolska G . SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019; 20(1). PMC: 6337402. DOI: 10.3390/ijms20010143. View

14.

Ding S, Zhang B, Qin F . Arabidopsis RZFP34/CHYR1, a Ubiquitin E3 Ligase, Regulates Stomatal Movement and Drought Tolerance via SnRK2.6-Mediated Phosphorylation. Plant Cell. 2015; 27(11):3228-44. PMC: 4682294. DOI: 10.1105/tpc.15.00321. View

15.

Zhang Y, Wan S, Liu X, He J, Cheng L, Duan M . Overexpression of CsSnRK2.5 increases tolerance to drought stress in transgenic Arabidopsis. Plant Physiol Biochem. 2020; 150:162-170. DOI: 10.1016/j.plaphy.2020.02.035. View

16.

Zhang H, Mao X, Wang C, Jing R . Overexpression of a common wheat gene TaSnRK2.8 enhances tolerance to drought, salt and low temperature in Arabidopsis. PLoS One. 2011; 5(12):e16041. PMC: 3012728. DOI: 10.1371/journal.pone.0016041. View

17.

Zhang H, Zhang X, Zhao J, Sun L, Wang H, Zhu Y . Genome-Wide Identification of GDSL-Type Esterase/Lipase Gene Family in L. Reveals That Is Related to BSMV Infection. Int J Mol Sci. 2021; 22(22). PMC: 8624868. DOI: 10.3390/ijms222212317. View

18.

Liu M, Li Y, Ma Y, Zhao Q, Stiller J, Feng Q . The draft genome of a wild barley genotype reveals its enrichment in genes related to biotic and abiotic stresses compared to cultivated barley. Plant Biotechnol J. 2019; 18(2):443-456. PMC: 6953193. DOI: 10.1111/pbi.13210. View

19.

Xing L, Qian C, Cao A, Li Y, Jiang Z, Li M . The Hv-SGT1 gene from Haynaldia villosa contributes to resistances towards both biotrophic and hemi-biotrophic pathogens in common wheat (Triticum aestivum L.). PLoS One. 2013; 8(9):e72571. PMC: 3760960. DOI: 10.1371/journal.pone.0072571. View

20.

Qi J, Song C, Wang B, Zhou J, Kangasjarvi J, Zhu J . Reactive oxygen species signaling and stomatal movement in plant responses to drought stress and pathogen attack. J Integr Plant Biol. 2018; 60(9):805-826. DOI: 10.1111/jipb.12654. View