Cloning of Eukaryotic Protein Synthesis Initiation Factor Genes: Isolation and Characterization of CDNA Clones Encoding Factor EIF-4A

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1985 Oct 11

PMID 3840589

Citations 51

Authors

P J Nielsen

G K McMaster

H Trachsel

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies directed against rabbit reticulocyte protein synthesis initiation factor 4A (eIF-4A) were used to isolate mouse cDNA clones expressing eIF-4A protein sequences in E. coli. The identity of cDNA clones encoding eIF-4A sequences was confirmed by hybrid-selected translation and peptide mapping of the translation product. Analysis of the mRNA coding for eIF-4A from mouse liver and HeLa cells by Northern hybridization revealed two discrete mRNA species of approximately 2000 and 1600 nucleotides in length. The existence of two mRNAs in mouse and HeLa cells encoding eIF-4A was confirmed by cDNA sequencing.

Citing Articles

Identification of a novel nuclease activity in human DDX49 helicase.

Parkes A, Anandavijayan S, Lou-Hing A, Downs O, Killelea T, Martin L R Soc Open Sci. 2024; 11(12):241891.

PMID: 39698160 PMC: 11651898. DOI: 10.1098/rsos.241891.

Phosphoglycerate dehydrogenase promotes pancreatic cancer development by interacting with eIF4A1 and eIF4E.

Ma X, Li B, Liu J, Fu Y, Luo Y J Exp Clin Cancer Res. 2019; 38(1):66.

PMID: 30744688 PMC: 6371491. DOI: 10.1186/s13046-019-1053-y.

Hippuristanol - A potent steroid inhibitor of eukaryotic initiation factor 4A.

Cencic R, Pelletier J Translation (Austin). 2016; 4(1):e1137381.

PMID: 27335721 PMC: 4909409. DOI: 10.1080/21690731.2015.1137381.

eIF4AII is dispensable for miRNA-mediated gene silencing.

Galicia-Vazquez G, Chu J, Pelletier J RNA. 2015; 21(10):1826-33.

PMID: 26286746 PMC: 4574758. DOI: 10.1261/rna.052225.115.

Targeting the eIF4F translation initiation complex: a critical nexus for cancer development.

Pelletier J, Graff J, Ruggero D, Sonenberg N Cancer Res. 2015; 75(2):250-63.

PMID: 25593033 PMC: 4299928. DOI: 10.1158/0008-5472.CAN-14-2789.

References

Anderson C, Baum P, GESTELAND R . Processing of adenovirus 2-induced proteins. J Virol. 1973; 12(2):241-52. PMC: 356618. DOI: 10.1128/JVI.12.2.241-252.1973. View

Biggin M, Gibson T, Hong G . Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983; 80(13):3963-5. PMC: 394179. DOI: 10.1073/pnas.80.13.3963. View

Cleveland D, Fischer S, Kirschner M, Laemmli U . Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977; 252(3):1102-6. View

Benne R, Luedi M, Hershey J . Purification and characterization of initiation factors IF-E4 and IF-E6 from rabbit reticulocytes. J Biol Chem. 1977; 252(16):5798-803. View

McMaster G, Carmichael G . Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977; 74(11):4835-8. PMC: 432050. DOI: 10.1073/pnas.74.11.4835. View

Trachsel H, Erni B, SCHREIER M, Staehelin T . Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors. J Mol Biol. 1977; 116(4):755-67. DOI: 10.1016/0022-2836(77)90269-8. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Buell G, Wickens M, Payvar F, Schimke R . Synthesis of full length cDNAs from four partially purified oviduct mRNAs. J Biol Chem. 1978; 253(7):2471-82. View

Revel M, Groner Y . Post-transcriptional and translational controls of gene expression in eukaryotes. Annu Rev Biochem. 1978; 47:1079-126. DOI: 10.1146/annurev.bi.47.070178.005243. View

10.

Safer B, Anderson W . The molecular mechanism of hemoglobin synthesis and its regulation in the reticulocyte. CRC Crit Rev Biochem. 1978; 5(4):261-90. DOI: 10.3109/10409237809177144. View

11.

Trachsel H, Erni B, SCHREIER M, Braun L, Staehelin T . Purification of seven protein synthesis initiation factors from Krebs II ascites cells. Biochim Biophys Acta. 1979; 561(2):484-90. DOI: 10.1016/0005-2787(79)90156-4. View

12.

Chamberlain J . Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979; 98(1):132-5. DOI: 10.1016/0003-2697(79)90716-4. View

13.

Schibler U, Tosi M, Pittet A, Fabiani L, Wellauer P . Tissue-specific expression of mouse alpha-amylase genes. J Mol Biol. 1980; 142(1):93-116. DOI: 10.1016/0022-2836(80)90208-9. View

14.

Hughes E, August J . Characterization of plasma membrane proteins identified by monoclonal antibodies. J Biol Chem. 1981; 256(2):664-71. View

15.

Oakley B, Kirsch D, Morris N . A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980; 105(2):361-3. DOI: 10.1016/0003-2697(80)90470-4. View

16.

Kozak M . Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981; 9(20):5233-52. PMC: 327517. DOI: 10.1093/nar/9.20.5233. View

17.

Grifo J, Tahara S, Leis J, Morgan M, Shatkin A, Merrick W . Characterization of eukaryotic initiation factor 4A, a protein involved in ATP-dependent binding of globin mRNA. J Biol Chem. 1982; 257(9):5246-52. View

18.

Maitra U, Stringer E, Chaudhuri A . Initiation factors in protein biosynthesis. Annu Rev Biochem. 1982; 51:869-900. DOI: 10.1146/annurev.bi.51.070182.004253. View

19.

Meyer L, Milburn S, Hershey J . Immunochemical characterization of mammalian protein synthesis initiation factors. Biochemistry. 1982; 21(18):4206-12. DOI: 10.1021/bi00261a003. View

20.

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View