Combining Near Infrared Fluorescence and Laser Speckle Imaging with Optical Tissue Clearing for in Vivo Transcranial Monitoring of Cerebral Blood Vessels Damaged by Photodynamic Nanoformulation

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2024 Feb 26

PMID 38404313

Authors

Jiantao Liu

Hao Xu

Siqi Gao

Liwei Liu

Junle Qu

Tymish Y Ohulchanskyy

Affiliations

Soon will be listed here.

Abstract

In vivo near infrared (NIR) fluorescence imaging and laser speckle contrast imaging (LSCI) are emerging optical bioimaging modalities, which can provide information on blood vessels morphology, volume and the blood flow velocity. Optical tissue clearing (OTC) technique addresses a light scattering problem in optical bioimaging, which is imperative for the transcranial brain imaging. Herein, we report an approach combining NIR fluorescence and LSC microscopy imaging with OTC. A liposomal nanoformulation comprising NIR fluorescent dye ICG and photosensitizer BPD was synthesized and injected intravenously into mouse with OTC treated skull. Transcranial excitation of BPD in nanoliposomes resulted in the localized, irradiation dose dependent photodynamic damage of the brain blood vessels, which was manifested both in NIR fluorescence and LSC transcranial imaging, revealing changes in the vessels morphology, volume and the blood flow rate. The developed approach allows for bimodal imaging guided, localized vascular PDT of cancer and other diseases.

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