Viral Interference Between the Insect-Specific Virus Brejeira and the Saint Louis Encephalitis Virus In Vitro

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2024 Feb 24

PMID 38399986

Authors

Ana Claudia Ribeiro

Livia Martins

Heloisa Silva

Maria Nazare Freitas

Maissa Santos

Ercilia Goncalves

Alana Sousa

Ivy Prazeres

Alessandra Santos

Ana Cecilia Cruz

Sandro Silva

Jannifer Chiang

Livia Casseb

Valeria Carvalho

Affiliations

Soon will be listed here.

Abstract

The Saint Louis encephalitis virus (SLEV) is an encephalitogenic arbovirus ( family) that has a wide geographical distribution in the western hemisphere, especially in the Americas. The negevirus Brejeira (BREV) was isolated for the first time in Brazil in 2005. This study aimed to verify the existence of a possible interfering effect of BREV on the course of SLEV infection and vice versa. We used clone C6/36 cells. Three combinations of MOIs were used (SLEV 0.1 × BREV 1; SLEV 1 × BREV 0.1; SLEV 1 × BREV 1) in the kinetics of up to 7 days and then the techniques of indirect immunofluorescence (IFA), a plaque assay on Vero cells, and RT-PCR were performed. Our results showed that the cytopathic effect (CPE) caused by BREV was more pronounced than the CPE caused by SLEV. Results of IFA, the plaque assay, and RT-PCR showed the suppression of SLEV replication in the co-infection condition in all the MOI combinations used. The SLEV suppression was dose-dependent. Therefore, the ISV Brejeira can suppress SLEV replication in cells, but SLEV does not negatively interfere with BREV replication.

References

Brackney D, Scott J, Sagawa F, Woodward J, Miller N, Schilkey F . C6/36 Aedes albopictus cells have a dysfunctional antiviral RNA interference response. PLoS Negl Trop Dis. 2010; 4(10):e856. PMC: 2964293. DOI: 10.1371/journal.pntd.0000856. View

Muckenfuss R . Clinical Observations and Laboratory Investigations on the 1933 Epidemic of Encephalitis in St. Louis. Bull N Y Acad Med. 2009; 10(7):444-53. PMC: 1965655. View

Ammerman N, Beier-Sexton M, Azad A . Growth and maintenance of Vero cell lines. Curr Protoc Microbiol. 2008; Appendix 4:Appendix 4E. PMC: 2657228. DOI: 10.1002/9780471729259.mca04es11. View

Baidaliuk A, Miot E, Lequime S, Moltini-Conclois I, Delaigue F, Dabo S . Cell-Fusing Agent Virus Reduces Arbovirus Dissemination in Aedes aegypti Mosquitoes . J Virol. 2019; 93(18). PMC: 6714787. DOI: 10.1128/JVI.00705-19. View

Hobson-Peters J, Yam A, Lu J, Setoh Y, May F, Kurucz N . A new insect-specific flavivirus from northern Australia suppresses replication of West Nile virus and Murray Valley encephalitis virus in co-infected mosquito cells. PLoS One. 2013; 8(2):e56534. PMC: 3584062. DOI: 10.1371/journal.pone.0056534. View

da Silva Ribeiro A, Martins L, da Silva S, Medeiros D, Miranda K, Neto J . Negeviruses isolated from mosquitoes in the Brazilian Amazon. Virol J. 2022; 19(1):17. PMC: 8778500. DOI: 10.1186/s12985-022-01743-z. View

Nunes M, Contreras-Gutierrez M, Guzman H, Martins L, Barbirato M, Savit C . Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. Virology. 2017; 504:152-167. PMC: 5394984. DOI: 10.1016/j.virol.2017.01.022. View

Hall R, Bielefeldt-Ohmann H, McLean B, OBrien C, Colmant A, Piyasena T . Commensal Viruses of Mosquitoes: Host Restriction, Transmission, and Interaction with Arboviral Pathogens. Evol Bioinform Online. 2017; 12(Suppl 2):35-44. PMC: 5226260. DOI: 10.4137/EBO.S40740. View

Vasconcelos P, da Rosa J, da Rosa A, Degallier N, Pinheiro F, Sa Filho G . [Epidemiology of encephalitis caused by arbovirus in the Brazilian Amazonia]. Rev Inst Med Trop Sao Paulo. 1991; 33(6):465-76. View

10.

Vasilakis N, Tesh R . Insect-specific viruses and their potential impact on arbovirus transmission. Curr Opin Virol. 2015; 15:69-74. PMC: 4688193. DOI: 10.1016/j.coviro.2015.08.007. View

11.

Goenaga S, Kenney J, Duggal N, Delorey M, Ebel G, Zhang B . Potential for Co-Infection of a Mosquito-Specific Flavivirus, Nhumirim Virus, to Block West Nile Virus Transmission in Mosquitoes. Viruses. 2015; 7(11):5801-12. PMC: 4664984. DOI: 10.3390/v7112911. View

12.

Erasmus J, Auguste A, Kaelber J, Luo H, Rossi S, Fenton K . A chikungunya fever vaccine utilizing an insect-specific virus platform. Nat Med. 2016; 23(2):192-199. PMC: 5296253. DOI: 10.1038/nm.4253. View

13.

Igarashi A . Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J Gen Virol. 1978; 40(3):531-44. DOI: 10.1099/0022-1317-40-3-531. View

14.

Nunes M, Silva S, Carvalho V, Vasconcelos J, Da Silva D, Oliveira L . Emergence of new insect-restrictive viruses in the Amazon region. Genome Announc. 2015; 3(2). PMC: 4395057. DOI: 10.1128/genomeA.00131-15. View

15.

Romo H, Kenney J, Blitvich B, Brault A . Restriction of Zika virus infection and transmission in Aedes aegypti mediated by an insect-specific flavivirus. Emerg Microbes Infect. 2018; 7(1):181. PMC: 6235874. DOI: 10.1038/s41426-018-0180-4. View

16.

Gubler D, Kuno G, SATHER G, Velez M, Oliver A . Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses. Am J Trop Med Hyg. 1984; 33(1):158-65. DOI: 10.4269/ajtmh.1984.33.158. View

17.

Kallies R, Kopp A, Zirkel F, Estrada A, Gillespie T, Drosten C . Genetic characterization of goutanap virus, a novel virus related to negeviruses, cileviruses and higreviruses. Viruses. 2014; 6(11):4346-57. PMC: 4246226. DOI: 10.3390/v6114346. View

18.

Blair C . Mosquito RNAi is the major innate immune pathway controlling arbovirus infection and transmission. Future Microbiol. 2011; 6(3):265-77. PMC: 3126673. DOI: 10.2217/fmb.11.11. View

19.

Diaz A, Coffey L, Burkett-Cadena N, Day J . Reemergence of St. Louis Encephalitis Virus in the Americas. Emerg Infect Dis. 2018; 24(12). PMC: 6256408. DOI: 10.3201/eid2412.180372. View

20.

Gorchakov R, Tesh R, Weaver S, Nasar F . Generation of an infectious Negev virus cDNA clone. J Gen Virol. 2014; 95(Pt 9):2071-2074. PMC: 4135092. DOI: 10.1099/vir.0.066019-0. View