Intestinal Effects of Filtered Alkalinized Water in Lean and Obese Zucker Rats

Overview

Journal Microorganisms

Specialty Microbiology

Date 2024 Feb 24

PMID 38399722

Authors

Laura Doblado

Ligia Esperanza Diaz

Esther Nova

Ascension Marcos

Maria Monsalve

Affiliations

Soon will be listed here.

Abstract

This study evaluated the intestinal effects of alkalinized filtered water in lean and obese adult Zucker rats. For 3 months, 12-week-old rats consumed either tap water or filtered alkalinized tap water from Madrid city. Weight gain was monitored, changes in metabolism were evaluated by indirect calorimetry, and total antioxidant capacity and levels of inflammatory mediators were measured in plasma. Feces were collected, their microbial composition was analyzed and histological analysis of the small and large intestine was performed, assessing the general state of the mucosa (MUC2), the inflammatory state (F4/80) and the presence of oxidative modifications in protein 4-Hydroxynonenal (4-HNE) by immunofluorescence (IF) and immunohistochemistry (IHC). The results obtained showed that the consumption of alkalinized filtered water improved the composition of the intestinal microbiome and the state of the intestinal mucosa, reducing both local and systemic inflammation and the level of oxidative stress. These changes were accompanied by a better maintenance of the oxidative status in rats. No differences were observed in antioxidant capacity nor in weight gain. The incorporation of probiotics in the diet had a significant impact on the microbiome. These effects were indicative of an improvement in general metabolic, oxidative and inflammatory status.

References

Gomes A, Hoffmann C, Mota J . The human gut microbiota: Metabolism and perspective in obesity. Gut Microbes. 2018; 9(4):308-325. PMC: 6219651. DOI: 10.1080/19490976.2018.1465157. View

Allin K, Tremaroli V, Caesar R, Jensen B, Damgaard M, Bahl M . Aberrant intestinal microbiota in individuals with prediabetes. Diabetologia. 2018; 61(4):810-820. PMC: 6448993. DOI: 10.1007/s00125-018-4550-1. View

Melnyk S, Hakkak R . Metabolic Status of Lean and Obese Zucker Rats Based on Untargeted and Targeted Metabolomics Analysis of Serum. Biomedicines. 2022; 10(1). PMC: 8773868. DOI: 10.3390/biomedicines10010153. View

Higashimura Y, Baba Y, Inoue R, Takagi T, Uchiyama K, Mizushima K . Effects of molecular hydrogen-dissolved alkaline electrolyzed water on intestinal environment in mice. Med Gas Res. 2018; 8(1):6-11. PMC: 5937304. DOI: 10.4103/2045-9912.229597. View

Armet A, Deehan E, OSullivan A, Mota J, Field C, Prado C . Rethinking healthy eating in light of the gut microbiome. Cell Host Microbe. 2022; 30(6):764-785. DOI: 10.1016/j.chom.2022.04.016. View

Luo Y, Lan C, Li H, Ouyang Q, Kong F, Wu A . Rational consideration of Akkermansia muciniphila targeting intestinal health: advantages and challenges. NPJ Biofilms Microbiomes. 2022; 8(1):81. PMC: 9576740. DOI: 10.1038/s41522-022-00338-4. View

Baldelli V, Scaldaferri F, Putignani L, Del Chierico F . The Role of Enterobacteriaceae in Gut Microbiota Dysbiosis in Inflammatory Bowel Diseases. Microorganisms. 2021; 9(4). PMC: 8066304. DOI: 10.3390/microorganisms9040697. View

Laursen M, Larsson M, Lind M, Larnkjaer A, Molgaard C, Michaelsen K . Intestinal Enterococcus abundance correlates inversely with excessive weight gain and increased plasma leptin in breastfed infants. FEMS Microbiol Ecol. 2020; 96(5). PMC: 7183236. DOI: 10.1093/femsec/fiaa066. View

Trakman G, Fehily S, Basnayake C, Hamilton A, Russell E, Wilson-OBrien A . Diet and gut microbiome in gastrointestinal disease. J Gastroenterol Hepatol. 2021; 37(2):237-245. DOI: 10.1111/jgh.15728. View

10.

Magne F, Gotteland M, Gauthier L, Zazueta A, Pesoa S, Navarrete P . The Firmicutes/Bacteroidetes Ratio: A Relevant Marker of Gut Dysbiosis in Obese Patients?. Nutrients. 2020; 12(5). PMC: 7285218. DOI: 10.3390/nu12051474. View

11.

Portincasa P, Khalil M, Graziani A, Fruhbeck G, Baffy G, Garruti G . Gut microbes in metabolic disturbances. Promising role for therapeutic manipulations?. Eur J Intern Med. 2023; 119:13-30. DOI: 10.1016/j.ejim.2023.10.002. View

12.

Hansen T, Thomassen M, Madsen M, Kern T, Bak E, Kashani A . The effect of drinking water pH on the human gut microbiota and glucose regulation: results of a randomized controlled cross-over intervention. Sci Rep. 2018; 8(1):16626. PMC: 6226457. DOI: 10.1038/s41598-018-34761-5. View

13.

Aziz I, Palsson O, Tornblom H, Sperber A, Whitehead W, Simren M . Epidemiology, clinical characteristics, and associations for symptom-based Rome IV functional dyspepsia in adults in the USA, Canada, and the UK: a cross-sectional population-based study. Lancet Gastroenterol Hepatol. 2018; 3(4):252-262. DOI: 10.1016/S2468-1253(18)30003-7. View

14.

Liu B, Liu X, Liang Z, Wang J . Gut microbiota in obesity. World J Gastroenterol. 2021; 27(25):3837-3850. PMC: 8291023. DOI: 10.3748/wjg.v27.i25.3837. View

15.

Yamawaki H, Futagami S, Wakabayashi M, Sakasegawa N, Agawa S, Higuchi K . Management of functional dyspepsia: state of the art and emerging therapies. Ther Adv Chronic Dis. 2018; 9(1):23-32. PMC: 5761940. DOI: 10.1177/2040622317725479. View

16.

Yibirin M, de Oliveira D, Valera R, Plitt A, Lutgen S . Adverse Effects Associated with Proton Pump Inhibitor Use. Cureus. 2021; 13(1):e12759. PMC: 7887997. DOI: 10.7759/cureus.12759. View

17.

Jin J, Cheng R, Ren Y, Shen X, Wang J, Xue Y . Distinctive Gut Microbiota in Patients with Overweight and Obesity with Dyslipidemia and its Responses to Long-term Orlistat and Ezetimibe Intervention: A Randomized Controlled Open-label Trial. Front Pharmacol. 2021; 12:732541. PMC: 8426637. DOI: 10.3389/fphar.2021.732541. View

18.

Guo P, Zhang K, Ma X, He P . species as probiotics: potentials and challenges. J Anim Sci Biotechnol. 2020; 11:24. PMC: 7031906. DOI: 10.1186/s40104-019-0402-1. View

19.

Wang R, Li Z, Liu S, Zhang D . Global, regional, and national burden of 10 digestive diseases in 204 countries and territories from 1990 to 2019. Front Public Health. 2023; 11:1061453. PMC: 10088561. DOI: 10.3389/fpubh.2023.1061453. View

20.

Ignacio A, Fernandes M, Rodrigues V, Groppo F, Cardoso A, Avila-Campos M . Correlation between body mass index and faecal microbiota from children. Clin Microbiol Infect. 2015; 22(3):258.e1-8. DOI: 10.1016/j.cmi.2015.10.031. View