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Effect of Low-Frequency Renal Nerve Stimulation on Renal Glucose Release During Normoglycemia and a Hypoglycemic Clamp in Pigs

Overview
Journal Int J Mol Sci
Publisher MDPI
Date 2024 Feb 24
PMID 38396718
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Abstract

Previously, we demonstrated that renal denervation in pigs reduces renal glucose release during a hypoglycemic episode. In this study we set out to examine changes in side-dependent renal net glucose release (SGN) through unilateral low-frequency stimulation (LFS) of the renal plexus with a pulse generator (2-5 Hz) during normoglycemia (60 min) and insulin-induced hypoglycemia ≤3.5 mmol/L (75 min) in seven pigs. The jugular vein, carotid artery, renal artery and vein, and both ureters were catheterized for measurement purposes, blood pressure management, and drug and fluid infusions. Para-aminohippurate (PAH) and inulin infusions were used to determine side-dependent renal plasma flow (SRP) and glomerular filtration rate (GFR). In a linear mixed model, LFS caused no change in SRP but decreased sodium excretion ( < 0.0001), as well as decreasing GFR during hypoglycemia ( = 0.0176). In a linear mixed model, only hypoglycemic conditions exerted significant effects on SGN ( = 0.001), whereas LFS did not. In a Wilcoxon signed rank exact test, LFS significantly increased SGN ( = 0.03125) and decreased sodium excretion ( = 0.0017) and urinary flow rate ( = 0.0129) when only considering the first instance LFS followed a preceding period of non-stimulation during normoglycemia. To conclude, this study represents, to our knowledge, the first description of an induction of renal gluconeogenesis by LFS.

References
1.
Kopp U . Role of renal sensory nerves in physiological and pathophysiological conditions. Am J Physiol Regul Integr Comp Physiol. 2014; 308(2):R79-95. PMC: 4297860. DOI: 10.1152/ajpregu.00351.2014. View

2.
Bohm M, Kario K, Kandzari D, Mahfoud F, Weber M, Schmieder R . Efficacy of catheter-based renal denervation in the absence of antihypertensive medications (SPYRAL HTN-OFF MED Pivotal): a multicentre, randomised, sham-controlled trial. Lancet. 2020; 395(10234):1444-1451. DOI: 10.1016/S0140-6736(20)30554-7. View

3.
Osborn J, Dibona G, Thames M . Beta-1 receptor mediation of renin secretion elicited by low-frequency renal nerve stimulation. J Pharmacol Exp Ther. 1981; 216(2):265-9. View

4.
Esler M, Rumantir M, Wiesner G, Kaye D, Hastings J, Lambert G . Sympathetic nervous system and insulin resistance: from obesity to diabetes. Am J Hypertens. 2001; 14(11 Pt 2):304S-309S. DOI: 10.1016/s0895-7061(01)02236-1. View

5.
Madhavan M, DeSimone C, Ebrille E, Mulpuru S, Mikell S, Johnson S . Transvenous stimulation of the renal sympathetic nerves increases systemic blood pressure: a potential new treatment option for neurocardiogenic syncope. J Cardiovasc Electrophysiol. 2014; 25(10):1115-8. PMC: 4447710. DOI: 10.1111/jce.12466. View