Phenotypic Investigation of Florfenicol Resistance and Molecular Detection of R Gene in Canine and Feline MDR Enterobacterales

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2024 Feb 23

PMID 38393089

Authors

Marios Lysitsas

Eleutherios Triantafillou

Vassiliki Spyrou

Charalambos Billinis

George Valiakos

Affiliations

Soon will be listed here.

Abstract

Florfenicol is a promising antibiotic for use in companion animals, especially as an alternative agent for infections caused by MDR bacteria. However, the emergence of resistant strains could hinder this potential. In this study, florfenicol resistance was investigated in a total of 246 MDR Enterobacterales obtained from canine and feline clinical samples in Greece over a two-year period (October 2020 to December 2022); a total of 44 (17,9%) florfenicol-resistant strains were recognized and further investigated. Most of these isolates originated from urine (41.9%) and soft tissue (37.2%) samples; ( = 14) and ( = 12) were the predominant species. The strains were examined for the presence of specific florfenicol-related resistance genes and In the majority of the isolates (31/44, 70.5%), the gene was detected, whereas none carried This finding creates concerns of co-acquisition of plasmid-mediated florfenicol-specific ARGs through horizontal transfer, along with several other resistance genes. The florfenicol resistance rates in MDR isolates seem relatively low but considerable for a second-line antibiotic; thus, in order to evaluate the potential of florfenicol to constitute an alternative antibiotic in companion animals, continuous monitoring of antibiotic resistance profiles is needed in order to investigate the distribution of florfenicol resistance under pressure of administration of commonly used agents.

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