Brown Algae Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis Through Heme Oxygenase-1 Activation in 3T3-L1 Cells

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2024 Feb 23

PMID 38393062

Authors

Lakshi A Dayarathne

Seok-Chun Ko

Mi-Jin Yim

Jeong Min Lee

Ji-Yul Kim

Gun-Woo Oh

Chul Hwan Kim

Kyung Woo Kim

Dae-Sung Lee

Jae-Young Je

Affiliations

Soon will be listed here.

Abstract

The present study aims to explore the probable anti-adipogenesis effect of () in 3T3-L1 preadipocytes by regulating heme oxygenase-1 (HO-1). The extract of retarded lipid accretion and decreased triglyceride (TG) content in 3T3-L1 adipocytes but increased free glycerol levels. Treatment with the extract inhibited lipogenesis by inhibiting protein expressions of fatty acid synthase (FAS) and lipoprotein lipase (LPL), whereas lipolysis increased by activating phosphorylation of hormone-sensitive lipase (p-HSL) and AMP-activated protein kinase (p-AMPK). The extract inhibited adipocyte differentiation of 3T3-L1 preadipocytes through down-regulating adipogenic transcription factors, including peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), and sterol regulatory element-binding protein 1 (SREBP1). This is attributed to the triggering of Wnt/β-catenin signaling. In addition, this study found that treatment with the extract activated HO-1 expression. Pharmacological approaches revealed that treatment with Zinc Protoporphyrin (ZnPP), an HO-1 inhibitor, resulted in an increase in lipid accumulation and a decrease in free glycerol levels. Finally, three adipogenic transcription factors, such as PPARγ, C/EBPα, and SREBP1, restored their expression in the presence of ZnPP. Analysis of chemical constituents revealed that the extract of is rich in 1,4-benzenediol, 7-tetradecenal, fucosterol, and n-hexadecanoic acid, which are known to have multiple pharmacological properties.

Citing Articles

Brown Algae Extract Modulates Adipogenesis and Browning in 3T3-L1 Preadipocytes through HO-1/Nrf2 Signaling.

Suryaningtyas I, Lee D, Je J Mar Drugs. 2024; 22(8).

PMID: 39195446 PMC: 11355876. DOI: 10.3390/md22080330.

Extract Suppresses Adiposity by Inhibiting Adipogenesis and Promoting Adipocyte Browning via AMPK Activation in 3T3-L1 Cells.

Dayarathne L, Jasmadi , Ko S, Yim M, Lee J, Kim J J Microbiol Biotechnol. 2024; 34(8):1688-1697.

PMID: 39086228 PMC: 11380521. DOI: 10.4014/jmb.2404.04041.

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