A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2024 Feb 23

PMID 38391981

Authors

Chengzhen Ji

Xuemei Tang

Ruiming Wen

Chengdong Xu

Jing Wei

Bingjun Han

Long Wu

Affiliations

Soon will be listed here.

Abstract

Ethephon (ETH), a commonly employed growth regulator, poses potential health risks due to its residue in fruits and vegetables, leading to both acute and subchronic toxicity. However, the detection accuracy of ETH is compromised by the color effects of the samples during the detection process. In this work, a multienzyme reaction-mediated electrochemical biosensor (MRMEC) was developed for the sensitive, rapid, and color-interference-resistant determination of ETH. Nanozymes FeO@Au-Pt and graphene nanocomplexes (GN-Au NPs) were prepared as catalysts and signal amplifiers for MRMEC. Acetylcholinesterase (AChE), acetylcholine (ACh), and choline oxidase (CHOx) form a cascade enzyme reaction to produce HO in an electrolytic cell. FeO@Au-Pt has excellent peroxidase-like activity and can catalyze the oxidation of 3,3',5,5'-tetramethvlbenzidine (TMB) in the presence of HO, resulting in a decrease in the characteristic peak current of TMB. Based on the inhibitory effect of ETH on AChE, the differential pulse voltammetry (DPV) current signal of TMB was used to detect ETH, offering the limit of detection (LOD) of 2.01 nmol L. The MRMEC method effectively analyzed ETH levels in mangoes, showing satisfactory precision (coefficient of variations, 2.88-15.97%) and recovery rate (92.18-110.72%). This biosensor holds promise for detecting various organophosphorus pesticides in food samples.

Citing Articles

A Comprehensive Review of Multifunctional Nanozymes for Degradation and Detection of Organophosphorus Pesticides in the Environment.

Liang J, Dong Z, Xu N, Chen T, Liang J, Xia M Toxics. 2025; 12(12.

PMID: 39771141 PMC: 11728651. DOI: 10.3390/toxics12120926.

Convenient and visual ethephon detection in fruits by enhanced fluorescence of metal-organic framework.

Zhang Z, Bai L, Han J, Li Z, Liu Q Mikrochim Acta. 2025; 192(1):55.

PMID: 39755984 DOI: 10.1007/s00604-024-06886-0.

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