Antimicrobial Resistance in Spp. Isolates from Red Foxes () in Latvia

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2024 Feb 23

PMID 38391500

Authors

Margarita Terentjeva

Juris kibilds

Jelena Avsejenko

Aivars Cirulis

Linda Labecka

Aivars Berzins

Affiliations

Soon will be listed here.

Abstract

Antimicrobial resistance (AMR) is an emerging public health threat and is one of the One Health priorities for humans, animals, and environmental health. Red foxes () are a widespread predator species with great ecological significance, and they may serve as a sentinel of antimicrobial resistance in the general environment. The present study was carried out to detect antimicrobial resistance, antimicrobial resistance genes, and genetic diversity in faecal isolates of red foxes (). In total, 34 isolates, including (n = 17), (n = 12), (n = 3), and (n = 2), were isolated. Antimicrobial resistance to 12 antimicrobial agents was detected with EUVENC panels using the minimum inhibitory concentration (MIC). The presence of antimicrobial resistance genes (ARGs) was determined using whole-genome sequencing (WGS). Resistance to tetracycline (6/34), erythromycin (3/34), ciprofloxacin (2/34), tigecycline (2/34), and daptomycin (2/34) was identified in 44% (15/34) of isolates, while all the isolates were found to be susceptible to ampicillin, chloramphenicol, gentamicin, linezolid, teicoplanin, and vancomycin. No multi-resistant spp. were detected. A total of 12 ARGs were identified in spp., with the presence of at least 1 ARG in every isolate. The identified ARGs encoded resistance to aminoglycosides (, , and ), tetracyclines (M), (L) and (S)), and macrolide-lincosamide-streptogramin AB ((B,G), (A,E), and (C)), and their presence was associated with phenotypical resistance. Core genome multilocus sequence typing (cgMLST) revealed the high diversity of and isolates, even within the same geographical area. The distribution of resistant spp. in wild foxes in Latvia highlights the importance of a One Health approach in tackling AMR.

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