Extremely Low-frequency Electromagnetic Fields Facilitate Both Osteoblast and Osteoclast Activity Through Wnt/β-catenin Signaling in the Zebrafish Scale

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2024 Feb 22

PMID 38385024

Authors

Jingjing Kobayashi-Sun

Isao Kobayashi

Makoto Kashima

Jun Hirayama

Makiko Kakikawa

Sotoshi Yamada

Nobuo Suzuki

Affiliations

Soon will be listed here.

Abstract

Electromagnetic fields (EMFs) have received widespread attention as effective, noninvasive, and safe therapies across a range of clinical applications for bone disorders. However, due to the various frequencies of devices, their effects on tissues/cells are vary, which has been a bottleneck in understanding the effects of EMFs on bone tissue. Here, we developed an model system using zebrafish scales to investigate the effects of extremely low-frequency EMFs (ELF-EMFs) on fracture healing. Exposure to 10 millitesla (mT) of ELF-EMFs at 60 Hz increased the number of both osteoblasts and osteoclasts in the fractured scale, whereas 3 or 30 mT did not. Gene expression analysis revealed that exposure to 10 mT ELF-EMFs upregulated and Wnt target genes in the fractured scale. Moreover, -catenin expression was enhanced by ELF-EMFs predominantly at the fracture site of the zebrafish scale. Inhibition of Wnt/-catenin signaling by IWR-1-endo treatment reduced both osteoblasts and osteoclasts in the fractured scale exposed to ELF-EMFs. These results suggest that ELF-EMFs promote both osteoblast and osteoclast activity through activation of Wnt/-catenin signaling in fracture healing. Our data provide evidence that ELF-EMFs generated with a widely used commercial AC power supply have a facilitative effect on fracture healing.

Citing Articles

Role of Pulsed Electromagnetic Field on Alveolar Bone Remodeling during Orthodontic Retention Phase in Rat Models.

Maulana H, Yueniwati Y, Permatasari N, Suyono H Dent J (Basel). 2024; 12(9).

PMID: 39329853 PMC: 11431648. DOI: 10.3390/dj12090287.

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