Bacterial Community and Genome Analysis of Cytoplasmic Incompatibility-inducing in American Serpentine Leafminer,

, an agricultural pest, is occasionally infected by A strain present in is associated with cytoplasmic incompatibility (CI) effects, leading to the death of embryos resulting from incompatible crosses between antibiotic-treated or naturally -free strain females and -infected males. In this study, high-throughput sequencing of hypervariable rRNA genes was employed to characterize the bacterial community in -infected without antibiotic treatment. The analysis revealed that dominates the bacterial community in , with minor presence of , , and . To elucidate the genetic basis of the CI phenotype, metagenomic sequencing was also conducted to assemble the genome of the strain. The draft-genome of the strain Ltri was 1.35 Mbp with 34% GC content and contained 1,487 predicted genes. Notably, within the Ltri genome, there are three distinct types of cytoplasmic incompatibility factor () genes: Type I, Type III, and Type V . These genes are likely responsible for inducing the strong cytoplasmic incompatibility observed in .