Impact of Physical Activity on Monocyte Subset CCR2 Expression and Macrophage Polarization Following Moderate Intensity Exercise

Overview

Journal Brain Behav Immun Health

Date 2024 Feb 20

PMID 38377416

Authors

Anson M Blanks

Thomas T Wagamon

Lindsay Lafratta

Mabel G Sisk

Morgan B Senter

Lauren N Pedersen

Natalie Bohmke

Attiya Shah

Virginia L Mihalick

R Lee Franco

Affiliations

Soon will be listed here.

Abstract

Purpose: To determine the impact of physical activity status on monocyte subset CCR2 surface expression and macrophage polarization in response to an acute bout of moderate intensity cycle ergometry.

Methods: 24 healthy women and men (12 high physically active [HIACT]: ≥1500 METmin/wk MVPA & 12 low physically active [LOACT]: <600 METmin/wk MVPA) underwent an acute moderate intensity (60% VO) bout of cycle ergometry for 30 min. Blood samples were collected prior to (PRE), immediately (POST), 1 h (1H), and 2 h (2H) following exercise. Monocyte CCR2 and macrophage CD86 (M1) and CD206 (M2) were analyzed by flow cytometry.

Results: Intermediate monocyte CCR2 decreased in response to exercise in the HIACT group (PRE: 11409.0 ± 1084.0 vs. POST: 9524.3 ± 1062.4; p = 0.034). Macrophage CD206 was lower in the LOACT compared to the HIACT group at 1H (HIACT: 67.2 ± 5.6 vs. LOACT: 50.1 ± 5.2%; p = 0.040). Macrophage CD206 at 1H was associated with both PRE (r = 0.446, p = 0.043) and POST (r = 0.464, p = 0.034) non-classical monocyte CCR2.

Conclusion: These data suggest that regular moderate to vigorous physical activity positively impacts both monocytes and macrophages following acute moderate intensity exercise and that this impact may contribute to the prevention of coronary artery disease.

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References

Tsianakas A, Varga G, Barczyk K, Bode G, Nippe N, Kran N . Induction of an anti-inflammatory human monocyte subtype is a unique property of glucocorticoids, but can be modified by IL-6 and IL-10. Immunobiology. 2011; 217(3):329-35. DOI: 10.1016/j.imbio.2011.10.002. View

Schild M, Eichner G, Beiter T, Zugel M, Krumholz-Wagner I, Hudemann J . Effects of Acute Endurance Exercise on Plasma Protein Profiles of Endurance-Trained and Untrained Individuals over Time. Mediators Inflamm. 2016; 2016:4851935. PMC: 4867072. DOI: 10.1155/2016/4851935. View

Agarwal S . Cardiovascular benefits of exercise. Int J Gen Med. 2012; 5:541-5. PMC: 3396114. DOI: 10.2147/IJGM.S30113. View

Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D . High pressure promotes monocyte adhesion to the vascular wall. Circ Res. 2007; 100(8):1226-33. DOI: 10.1161/01.RES.0000265231.59354.2c. View

Sierra-Filardi E, Nieto C, Dominguez-Soto A, Barroso R, Sanchez-Mateos P, Puig-Kroger A . CCL2 shapes macrophage polarization by GM-CSF and M-CSF: identification of CCL2/CCR2-dependent gene expression profile. J Immunol. 2014; 192(8):3858-67. DOI: 10.4049/jimmunol.1302821. View

Wong K, Tai J, Wong W, Han H, Sem X, Yeap W . Gene expression profiling reveals the defining features of the classical, intermediate, and nonclassical human monocyte subsets. Blood. 2011; 118(5):e16-31. DOI: 10.1182/blood-2010-12-326355. View

Peake J, Neubauer O, Della Gatta P, Nosaka K . Muscle damage and inflammation during recovery from exercise. J Appl Physiol (1985). 2016; 122(3):559-570. DOI: 10.1152/japplphysiol.00971.2016. View

Cabral-Santos C, de Lima Junior E, Fernandes I, Pinto R, Rosa-Neto J, Bishop N . Interleukin-10 responses from acute exercise in healthy subjects: A systematic review. J Cell Physiol. 2018; 234(7):9956-9965. DOI: 10.1002/jcp.27920. View

Ruffino J, Davies N, Morris K, Ludgate M, Zhang L, Webb R . Moderate-intensity exercise alters markers of alternative activation in circulating monocytes in females: a putative role for PPARγ. Eur J Appl Physiol. 2016; 116(9):1671-82. PMC: 4983283. DOI: 10.1007/s00421-016-3414-y. View

10.

Schembre S, Riebe D . Non-exercise estimation of VO(2)max using the International Physical Activity Questionnaire. Meas Phys Educ Exerc Sci. 2011; 15(3):168-181. PMC: 3173948. DOI: 10.1080/1091367X.2011.568369. View

11.

Volpe S, Cameroni E, Moepps B, Thelen S, Apuzzo T, Thelen M . CCR2 acts as scavenger for CCL2 during monocyte chemotaxis. PLoS One. 2012; 7(5):e37208. PMC: 3355119. DOI: 10.1371/journal.pone.0037208. View

12.

Bartoli C, Civatte M, Pellissier J, Figarella-Branger D . CCR2A and CCR2B, the two isoforms of the monocyte chemoattractant protein-1 receptor are up-regulated and expressed by different cell subsets in idiopathic inflammatory myopathies. Acta Neuropathol. 2001; 102(4):385-92. DOI: 10.1007/s004010100394. View

13.

Timmerman K, Flynn M, Coen P, Markofski M, Pence B . Exercise training-induced lowering of inflammatory (CD14+CD16+) monocytes: a role in the anti-inflammatory influence of exercise?. J Leukoc Biol. 2008; 84(5):1271-8. DOI: 10.1189/jlb.0408244. View

14.

Fredsted A, Clausen T, Overgaard K . Effects of step exercise on muscle damage and muscle Ca2+ content in men and women. J Strength Cond Res. 2008; 22(4):1136-46. DOI: 10.1519/JSC.0b013e318173db9b. View

15.

Zhang Y, Johnson 2nd M, Chow N, Wasserman K . Effect of exercise testing protocol on parameters of aerobic function. Med Sci Sports Exerc. 1991; 23(5):625-30. View

16.

Kawanishi N, Yano H, Yokogawa Y, Suzuki K . Exercise training inhibits inflammation in adipose tissue via both suppression of macrophage infiltration and acceleration of phenotypic switching from M1 to M2 macrophages in high-fat-diet-induced obese mice. Exerc Immunol Rev. 2010; 16:105-18. View

17.

Dhabhar F . Stress-induced augmentation of immune function--the role of stress hormones, leukocyte trafficking, and cytokines. Brain Behav Immun. 2002; 16(6):785-98. DOI: 10.1016/s0889-1591(02)00036-3. View

18.

Walton R, Kosmac K, Mula J, Fry C, Peck B, Groshong J . Human skeletal muscle macrophages increase following cycle training and are associated with adaptations that may facilitate growth. Sci Rep. 2019; 9(1):969. PMC: 6353900. DOI: 10.1038/s41598-018-37187-1. View

19.

Moore K, Sheedy F, Fisher E . Macrophages in atherosclerosis: a dynamic balance. Nat Rev Immunol. 2013; 13(10):709-21. PMC: 4357520. DOI: 10.1038/nri3520. View

20.

Fantuzzi L, Borghi P, Ciolli V, Pavlakis G, Belardelli F, Gessani S . Loss of CCR2 expression and functional response to monocyte chemotactic protein (MCP-1) during the differentiation of human monocytes: role of secreted MCP-1 in the regulation of the chemotactic response. Blood. 1999; 94(3):875-83. View