In-situ Gelation of Fibrin Gel Encapsulating Platelet-rich Plasma-derived Exosomes Promotes Rotator Cuff Healing

Overview

Journal Commun Biol

Specialty Biology

Date 2024 Feb 20

PMID 38374439

Authors

Molin Li

Lin Shi

Xianghui Chen

Dan Yi

Yufei Ding

Jian Chen

Guanghui Xing

Siming Chen

Li Wang

Yongyi Zhang

Yaqiong Zhu

Yuexiang Wang

Affiliations

Soon will be listed here.

Abstract

Although platelet-rich plasma-derived exosomes (PRP-Exos) hold significant repair potential, their efficacy in treating rotator cuff tear (RCT) remains unknown. In light of the potential for clinical translation of fibrin gel and PRP-Exos, we evaluated their combined impact on RCT healing and explored suitable gel implantation techniques. In vitro experiments demonstrated that PRP-Exos effectively enhanced key phenotypes changes in tendon stem/progenitor cells. Multi-modality imaging, including conventional ultrasound, shear wave elastography ultrasound, and micro-computed tomography, and histopathological assessments were performed to collectively evaluate the regenerative effects on RCT. The regenerated tendons exhibited a well-ordered structure, while bone and cartilage regeneration were significantly improved. PRP-Exos participated in the healing process of RCT. In-situ gelation of fibrin gel-encapsulated PRP-Exos at the bone-tendon interface during surgery proved to be a feasible gel implantation method that benefits the healing outcome. Comprehensive multi-modality postoperative evaluations were necessary, providing a reliable foundation for post-injury repair.

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