Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis

Overview

Journal Anal Chem

Specialty Chemistry

Date 2024 Feb 19

PMID 38372289

Authors

Jayden Lee Roberts

Luke Whiley

Nicola Gray

Melvin Gay

Philipp Nitschke

Reika Masuda

Elaine Holmes

Jeremy K Nicholson

Julien Wist

Nathan G Lawler

Affiliations

Soon will be listed here.

Abstract

We investigated plasma and serum blood derivatives from capillary blood microsamples (500 μL, MiniCollect tubes) and corresponding venous blood (10 mL vacutainers). Samples from 20 healthy participants were analyzed by H NMR, and 112 lipoprotein subfraction parameters; 3 supramolecular phospholipid composite (SPC) parameters from SPC, SPC, and SPC subfractions; 2 -acetyl signals from α-1-acid glycoprotein (Glyc), GlycA, and GlycB; and 3 calculated parameters, SPC (total), SPC/SPC, and Glyc (total) were assessed. Using linear regression between capillary and venous collection sites, we explained that agreement (Adj. ≥ 0.8, < 0.001) was witnessed for 86% of plasma parameters (103/120) and 88% of serum parameters (106/120), indicating that capillary lipoprotein, SPC, and Glyc concentrations follow changes in venous concentrations. These results indicate that capillary blood microsamples are suitable for sampling in remote areas and for high-frequency longitudinal sampling of the majority of lipoproteins, SPCs, and Glycs.

Citing Articles

Cross-Validation of Metabolic Phenotypes in SARS-CoV-2 Infected Subpopulations Using Targeted Liquid Chromatography-Mass Spectrometry (LC-MS).

Whiley L, Lawler N, Zeng A, Lee A, Chin S, Bizkarguenaga M J Proteome Res. 2024; 23(4):1313-1327.

PMID: 38484742 PMC: 11002931. DOI: 10.1021/acs.jproteome.3c00797.

References

Anderson N, Anderson N . The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics. 2002; 1(11):845-67. DOI: 10.1074/mcp.r200007-mcp200. View

Lodge S, Lawler N, Gray N, Masuda R, Nitschke P, Whiley L . Integrative Plasma Metabolic and Lipidomic Modelling of SARS-CoV-2 Infection in Relation to Clinical Severity and Early Mortality Prediction. Int J Mol Sci. 2023; 24(14). PMC: 10380980. DOI: 10.3390/ijms241411614. View

Kimhofer T, Lodge S, Whiley L, Gray N, Loo R, Lawler N . Integrative Modeling of Quantitative Plasma Lipoprotein, Metabolic, and Amino Acid Data Reveals a Multiorgan Pathological Signature of SARS-CoV-2 Infection. J Proteome Res. 2020; 19(11):4442-4454. DOI: 10.1021/acs.jproteome.0c00519. View

Lamarche B, Tchernof A, Moorjani S, Cantin B, Dagenais G, Lupien P . Small, dense low-density lipoprotein particles as a predictor of the risk of ischemic heart disease in men. Prospective results from the Québec Cardiovascular Study. Circulation. 1997; 95(1):69-75. DOI: 10.1161/01.cir.95.1.69. View

Nicholson J, OFlynn M, Sadler P, Macleod A, Juul S, Sonksen P . Proton-nuclear-magnetic-resonance studies of serum, plasma and urine from fasting normal and diabetic subjects. Biochem J. 1984; 217(2):365-75. PMC: 1153226. DOI: 10.1042/bj2170365. View

Bell J, Brown J, Nicholson J, Sadler P . Assignment of resonances for 'acute-phase' glycoproteins in high resolution proton NMR spectra of human blood plasma. FEBS Lett. 1987; 215(2):311-5. DOI: 10.1016/0014-5793(87)80168-0. View

Rossler T, Berezhnoy G, Singh Y, Cannet C, Reinsperger T, Schafer H . Quantitative Serum NMR Spectroscopy Stratifies COVID-19 Patients and Sheds Light on Interfaces of Host Metabolism and the Immune Response with Cytokines and Clinical Parameters. Metabolites. 2022; 12(12). PMC: 9781847. DOI: 10.3390/metabo12121277. View

Okami Y, Chan Q, Miura K, Kadota A, Elliott P, Masaki K . Small High-Density Lipoprotein and Omega-3 Fatty Acid Intake Differentiates Japanese and Japanese-Americans: The INTERLIPID Study. J Atheroscler Thromb. 2022; 30(8):884-906. PMC: 10406687. DOI: 10.5551/jat.63762. View

Nitschke P, Lodge S, Hall D, Schaefer H, Spraul M, Embade N . Direct low field J-edited diffusional proton NMR spectroscopic measurement of COVID-19 inflammatory biomarkers in human serum. Analyst. 2022; 147(19):4213-4221. DOI: 10.1039/d2an01097f. View

10.

Masuda R, Lodge S, Whiley L, Gray N, Lawler N, Nitschke P . Exploration of Human Serum Lipoprotein Supramolecular Phospholipids Using Statistical Heterospectroscopy in -Dimensions (SHY-): Identification of Potential Cardiovascular Risk Biomarkers Related to SARS-CoV-2 Infection. Anal Chem. 2022; 94(10):4426-4436. DOI: 10.1021/acs.analchem.1c05389. View

11.

Yuan L, Li-Gao R, Verhoeven A, van Eyk H, Bizino M, Rensen P . Altered high-density lipoprotein composition is associated with risk for complications in type 2 diabetes mellitus in South Asian descendants: A cross-sectional, case-control study on lipoprotein subclass profiling. Diabetes Obes Metab. 2023; 25(8):2374-2387. DOI: 10.1111/dom.15118. View

12.

Mallagaray A, Rudolph L, Lindloge M, Molbitz J, Thomsen H, Schmelter F . Towards a Precise NMR Quantification of Acute Phase Inflammation Proteins from Human Serum. Angew Chem Int Ed Engl. 2023; 62(35):e202306154. DOI: 10.1002/anie.202306154. View

13.

Hood L . Systems biology and p4 medicine: past, present, and future. Rambam Maimonides Med J. 2013; 4(2):e0012. PMC: 3678833. DOI: 10.5041/RMMJ.10112. View

14.

Kazenwadel J, Berezhnoy G, Cannet C, Schafer H, Geisler T, Rohlfing A . Stratification of hypertension and SARS-CoV-2 infection by quantitative NMR spectroscopy of human blood serum. Commun Med (Lond). 2023; 3(1):145. PMC: 11081957. DOI: 10.1038/s43856-023-00365-y. View

15.

Stanciulescu L, Scafa A, Duduianu C, Stan R, Nicolescu A, Deleanu C . Lipoprofiling Assessed by NMR Spectroscopy in Patients with Acute Coronary Syndromes: Is There a Need for Fasting Prior to Sampling?. Diagnostics (Basel). 2022; 12(7). PMC: 9319954. DOI: 10.3390/diagnostics12071675. View

16.

Loo R, Lodge S, Kimhofer T, Bong S, Begum S, Whiley L . Quantitative In-Vitro Diagnostic NMR Spectroscopy for Lipoprotein and Metabolite Measurements in Plasma and Serum: Recommendations for Analytical Artifact Minimization with Special Reference to COVID-19/SARS-CoV-2 Samples. J Proteome Res. 2020; 19(11):4428-4441. DOI: 10.1021/acs.jproteome.0c00537. View

17.

Kupke I, Kather B, Zeugner S . On the composition of capillary and venous blood serum. Clin Chim Acta. 1981; 112(2):177-85. DOI: 10.1016/0009-8981(81)90376-4. View

18.

Cutler D . The Costs of Long COVID. JAMA Health Forum. 2022; 3(5):e221809. DOI: 10.1001/jamahealthforum.2022.1809. View

19.

Mallol R, Rodriguez M, Brezmes J, Masana L, Correig X . Human serum/plasma lipoprotein analysis by NMR: application to the study of diabetic dyslipidemia. Prog Nucl Magn Reson Spectrosc. 2013; 70:1-24. DOI: 10.1016/j.pnmrs.2012.09.001. View

20.

Otvos J, Shalaurova I, Wolak-Dinsmore J, Connelly M, Mackey R, Stein J . GlycA: A Composite Nuclear Magnetic Resonance Biomarker of Systemic Inflammation. Clin Chem. 2015; 61(5):714-23. DOI: 10.1373/clinchem.2014.232918. View