In Vitro Enhanced Osteogenic Potential of Human Mesenchymal Stem Cells Seeded in a Poly (Lactic--Glycolic) Acid Scaffold: A Systematic Review

Overview

Journal Craniomaxillofac Trauma Reconstr

Publisher Sage Publications

Date 2024 Feb 19

PMID 38371215

Authors

Karla C Maita

Francisco R Avila

Ricardo A Torres-Guzman

Rachel Sarabia-Estrada

Abba C Zubair

Alfredo Quinones-Hinojosa

Antonio J Forte

Affiliations

Soon will be listed here.

Abstract

Study Design: Human bone marrow stem cells (hBMSCs) and human adipose-derived stem cells (hADSCs) have demonstrated the capability to regenerate bone once they have differentiated into osteoblasts.

Objective: This systematic review aimed to evaluate the in vitro osteogenic differentiation potential of these cells when seeded in a poly (lactic--glycolic) acid (PLGA) scaffold.

Methods: A literature search of 4 databases following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines was conducted in January 2021 for studies evaluating the osteogenic differentiation potential of hBMSCs and hADSCs seeded in a PLGA scaffold. Only in vitro models were included. Studies in languages other than English were excluded.

Results: A total of 257 studies were identified after the removal of duplicates. Seven articles fulfilled our inclusion and exclusion criteria. Four of these reviews used hADSCs and three used hBMSCs in the scaffold. Upregulation in osteogenic gene expression was seen in all the cells seeded in a 3-dimensional scaffold compared with 2-dimensional films. High angiogenic gene expression was found in hADSCs. Addition of inorganic material to the scaffold material affected cell performance.

Conclusions: Viability, proliferation, and differentiation of cells strongly depend on the environment where they grow. There are several factors that can enhance the differentiation capacity of stem cells. A PLGA scaffold proved to be a biocompatible material capable of boosting the osteogenic differentiation potential and mineralization capacity in hBMSCs and hADSCs.

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References

Crist T, Mathew P, Plotsker E, Sevilla A, Thaller S . Biomaterials in Craniomaxillofacial Reconstruction: Past, Present, and Future. J Craniofac Surg. 2021; 32(2):535-540. DOI: 10.1097/SCS.0000000000007079. View

Du B, Yin H, Chen Y, Lin W, Wang Y, Zhao D . A waterborne polyurethane 3D scaffold containing PLGA with a controllable degradation rate and an anti-inflammatory effect for potential applications in neural tissue repair. J Mater Chem B. 2020; 8(20):4434-4446. DOI: 10.1039/d0tb00656d. View

Zhao B, Xu H, Gao Y, Xu J, Yin H, Xu L . Promoting osteoblast proliferation on polymer bone substitutes with bone-like structure by combining hydroxyapatite and bioactive glass. Mater Sci Eng C Mater Biol Appl. 2019; 96:1-9. DOI: 10.1016/j.msec.2018.11.006. View

Ho-Shui-Ling A, Bolander J, Rustom L, Johnson A, Luyten F, Picart C . Bone regeneration strategies: Engineered scaffolds, bioactive molecules and stem cells current stage and future perspectives. Biomaterials. 2018; 180:143-162. PMC: 6710094. DOI: 10.1016/j.biomaterials.2018.07.017. View

Groninger O, Hess S, Mohn D, Schneider E, Stark W, Marsmann S . Directing Stem Cell Commitment by Amorphous Calcium Phosphate Nanoparticles Incorporated in PLGA: Relevance of the Free Calcium Ion Concentration. Int J Mol Sci. 2020; 21(7). PMC: 7177725. DOI: 10.3390/ijms21072627. View

Sterne J, Hernan M, Reeves B, Savovic J, Berkman N, Viswanathan M . ROBINS-I: a tool for assessing risk of bias in non-randomised studies of interventions. BMJ. 2016; 355:i4919. PMC: 5062054. DOI: 10.1136/bmj.i4919. View

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D . Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-7. DOI: 10.1080/14653240600855905. View

Zong C, Xue D, Yuan W, Wang W, Shen D, Tong X . Reconstruction of rat calvarial defects with human mesenchymal stem cells and osteoblast-like cells in poly-lactic-co-glycolic acid scaffolds. Eur Cell Mater. 2011; 20:109-20. DOI: 10.22203/ecm.v020a10. View

Kinaci A, Neuhaus V, Ring D . Trends in bone graft use in the United States. Orthopedics. 2014; 37(9):e783-8. DOI: 10.3928/01477447-20140825-54. View

10.

Pereira H, Cengiz I, Silva F, Reis R, Oliveira J . Scaffolds and coatings for bone regeneration. J Mater Sci Mater Med. 2020; 31(3):27. DOI: 10.1007/s10856-020-06364-y. View

11.

Huang C, Huang W, Zuk P, Jarrahy R, Rudkin G, Ishida K . Genetic markers of osteogenesis and angiogenesis are altered in processed lipoaspirate cells when cultured on three-dimensional scaffolds. Plast Reconstr Surg. 2008; 121(2):411-423. DOI: 10.1097/01.prs.0000298510.03226.5f. View

12.

Kern S, Eichler H, Stoeve J, Kluter H, Bieback K . Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-301. DOI: 10.1634/stemcells.2005-0342. View

13.

FRIEDENSTEIN A, PETRAKOVA K, Kurolesova A, Frolova G . Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 1968; 6(2):230-47. View

14.

Bhuiyan D, Middleton J, Tannenbaum R, Wick T . Bone regeneration from human mesenchymal stem cells on porous hydroxyapatite-PLGA-collagen bioactive polymer scaffolds. Biomed Mater Eng. 2017; 28(6):671-685. DOI: 10.3233/BME-171703. View

15.

Mokhtari-Jafari F, Amoabediny G, Dehghan M . Role of biomechanics in vascularization of tissue-engineered bones. J Biomech. 2020; 110:109920. DOI: 10.1016/j.jbiomech.2020.109920. View

16.

Park S, Sim W, Min B, Yang S, Khademhosseini A, Kaplan D . Chip-based comparison of the osteogenesis of human bone marrow- and adipose tissue-derived mesenchymal stem cells under mechanical stimulation. PLoS One. 2012; 7(9):e46689. PMC: 3460891. DOI: 10.1371/journal.pone.0046689. View

17.

Qi H, Ye Z, Ren H, Chen N, Zeng Q, Wu X . Bioactivity assessment of PLLA/PCL/HAP electrospun nanofibrous scaffolds for bone tissue engineering. Life Sci. 2016; 148:139-44. DOI: 10.1016/j.lfs.2016.02.040. View

18.

Im G, Shin Y, Lee K . Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells?. Osteoarthritis Cartilage. 2005; 13(10):845-53. DOI: 10.1016/j.joca.2005.05.005. View

19.

Mizuno H, Tobita M, Uysal A . Concise review: Adipose-derived stem cells as a novel tool for future regenerative medicine. Stem Cells. 2012; 30(5):804-10. DOI: 10.1002/stem.1076. View

20.

Zuk P, Zhu M, Mizuno H, Huang J, Futrell J, Katz A . Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001; 7(2):211-28. DOI: 10.1089/107632701300062859. View