Ligase-mediated Synthesis of Cu-responsive Allosteric DNAzyme with Bifacial 5-carboxyuracil Nucleobases

Overview

Journal Chem Sci

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2024 Feb 16

PMID 38362437

Authors

Yusuke Takezawa

Hanci Zhang

Keita Mori

Lingyun Hu

Mitsuhiko Shionoya

Affiliations

Soon will be listed here.

Abstract

A Cu-responsive allosteric DNAzyme has been developed by introducing bifacial 5-carboxyuracil (caU) nucleobases, which form both hydrogen-bonded caU-A and metal-mediated caU-Cu-caU base pairs. The base sequence was logically designed based on a known RNA-cleaving DNAzyme so that the caU-modified DNAzyme (caU-DNAzyme) can form a catalytically inactive structure containing three caU-A base pairs and an active form with three caU-Cu-caU pairs. The caU-DNAzyme was synthesized by joining short caU-containing fragments with a standard DNA ligase. The activity of caU-DNAzyme was suppressed without Cu, but enhanced 21-fold with the addition of Cu. Furthermore, the DNAzyme activity was turned on and off during the reaction by the addition and removal of Cu ions. Both ligase-mediated synthesis and Cu-dependent allosteric regulation were achieved by the bifacial base pairing properties of caU. This study provides a new strategy for designing stimuli-responsive DNA molecular systems.

Citing Articles

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Pichon M, Hollenstein M Commun Chem. 2024; 7(1):138.

PMID: 38890393 PMC: 11189433. DOI: 10.1038/s42004-024-01216-0.

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