The Recombinant L-lysine α-oxidase from the Fungus Trichoderma Harzianum Promotes Apoptosis and Necrosis of Leukemia CD34 + hematopoietic Cells

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2024 Feb 14

PMID 38355518

Authors

Mariana do Nascimento Costa

Thiago Aparecido Silva

Dimitrius Santiago Passos Simoes Froes Guimaraes

Rafael Ricci-Azevedo

Felipe Roberti Teixeira

Leonardo Reis Silveira

Marcelo Damario Gomes

Vitor Marcel Faca

Eduardo Brandt de Oliveira

Rodrigo T Calado

Roberto N Silva

Affiliations

Soon will be listed here.

Abstract

Background: In hematologic cancers, including leukemia, cells depend on amino acids for rapid growth. Anti-metabolites that prevent their synthesis or promote their degradation are considered potential cancer treatment agents. Amino acid deprivation triggers proliferation inhibition, autophagy, and programmed cell death. L-lysine, an essential amino acid, is required for tumor growth and has been investigated for its potential as a target for cancer treatment. L-lysine α-oxidase, a flavoenzyme that degrades L-lysine, has been studied for its ability to induce apoptosis and prevent cancer cell proliferation. In this study, we describe the use of L-lysine α-oxidase (LO) from the filamentous fungus Trichoderma harzianum for cancer treatment.

Results: The study identified and characterized a novel LO from T. harzianum and demonstrated that the recombinant protein (rLO) has potent and selective cytotoxic effects on leukemic cells by triggering the apoptotic cascade through mitochondrial dysfunction.

Conclusions: The results support future translational studies using the recombinant LO as a potential drug for the treatment of leukemia.

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