Study on Seed-borne Cultivable Bacterial Diversity and Antibiotic Resistance of L

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Feb 14

PMID 38351918

Authors

Jinjing Xie

Jie Yang

Shaowei Zhu

Xuan Hou

Haiyan Chen

Xiaoming Bai

Zhenfen Zhang

Affiliations

Soon will be listed here.

Abstract

In order to study the difference of cultivable seed-borne bacterial diversity between commercial varieties and wild species of L., and their antibiotic resistance to sulfadiazine, tetracycline, oxytetracycline, ciprofloxacin, gentamicin, oxytetracycline and rifampin. In this study, 60 bacterium isolates were isolated by dilution-coated plate method. Through 16S rRNA sequence analysis, 40 representative isolates with different morphological characteristics were identified and phylogenetic tree was constructed. The results of diversity analysis showed that the seed-borne bacterial diversity of commercial varieties was richer than that of wild species. The antibiotic resistance of the isolated bacterial strains was studied by agar dilution method, and it was concluded that the antibiotic resistance of the seed-borne bacteria carried by commercial varieties was stronger than that of the wild species. Finally, the biofilm formation ability and swimming motility of the bacterial strain were measured, and the correlation between the two and the antibiotic resistance of the bacterial strain was analyzed. The analysis showed that the antibiotic resistance of bacterial strains in L. was significantly correlated with their swimming motility. In addition, the swimming motility of the bacterial strains was significantly correlated with the biofilm formation ability. It is worth mentioning that this is the first time to study the drug-resistant bacteria distributed in the seed-borne bacteria of L.

References

Penesyan A, Paulsen I, Kjelleberg S, Gillings M . Three faces of biofilms: a microbial lifestyle, a nascent multicellular organism, and an incubator for diversity. NPJ Biofilms Microbiomes. 2021; 7(1):80. PMC: 8581019. DOI: 10.1038/s41522-021-00251-2. View

Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I . Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007; 115(8):891-9. DOI: 10.1111/j.1600-0463.2007.apm_630.x. View

Compant S, Mitter B, Colli-Mull J, Gangl H, Sessitsch A . Endophytes of grapevine flowers, berries, and seeds: identification of cultivable bacteria, comparison with other plant parts, and visualization of niches of colonization. Microb Ecol. 2011; 62(1):188-97. DOI: 10.1007/s00248-011-9883-y. View

Hoiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O . Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents. 2010; 35(4):322-32. DOI: 10.1016/j.ijantimicag.2009.12.011. View

Cottyn B, Regalado E, Lanoot B, De Cleene M, Mew T, Swings J . Bacterial populations associated with rice seed in the tropical environment. Phytopathology. 2008; 91(3):282-92. DOI: 10.1094/PHYTO.2001.91.3.282. View

Muola A, Fuchs B, Laihonen M, Rainio K, Heikkonen L, Ruuskanen S . Risk in the circular food economy: Glyphosate-based herbicide residues in manure fertilizers decrease crop yield. Sci Total Environ. 2020; 750:141422. DOI: 10.1016/j.scitotenv.2020.141422. View

Tian X, Song Z, Suo B, Zhou L, Li C, Zhang Y . [Comparison of Epsilometer test and agar dilution method in detecting the sensitivity of to metronidazole]. Beijing Da Xue Xue Bao Yi Xue Ban. 2023; 55(5):934-938. PMC: 10560899. View

Zheng S, Bawazir M, Dhall A, Kim H, He L, Heo J . Implication of Surface Properties, Bacterial Motility, and Hydrodynamic Conditions on Bacterial Surface Sensing and Their Initial Adhesion. Front Bioeng Biotechnol. 2021; 9:643722. PMC: 7907602. DOI: 10.3389/fbioe.2021.643722. View

Zhou P, Chen Y, Lu Q, Qin H, Ou H, He B . Cellular metabolism network of Bacillus thuringiensis related to erythromycin stress and degradation. Ecotoxicol Environ Saf. 2018; 160:328-341. DOI: 10.1016/j.ecoenv.2018.05.048. View

10.

Palma V, Gutierrez M, Vargas O, Parthasarathy R, Navarrete P . Methods to Evaluate Bacterial Motility and Its Role in Bacterial-Host Interactions. Microorganisms. 2022; 10(3). PMC: 8953368. DOI: 10.3390/microorganisms10030563. View

11.

Nagasawa R, Sato T, Nomura N, Nakamura T, Senpuku H . Potential Risk of Spreading Resistance Genes within Extracellular-DNA-Dependent Biofilms of Streptococcus mutans in Response to Cell Envelope Stress Induced by Sub-MICs of Bacitracin. Appl Environ Microbiol. 2020; 86(16). PMC: 7414966. DOI: 10.1128/AEM.00770-20. View

12.

Fierer N, Leff J, Adams B, Nielsen U, Bates S, Lauber C . Cross-biome metagenomic analyses of soil microbial communities and their functional attributes. Proc Natl Acad Sci U S A. 2012; 109(52):21390-5. PMC: 3535587. DOI: 10.1073/pnas.1215210110. View

13.

Jogaiah S, Kurjogi M, Govind S, Huntrike S, Basappa V, Tran L . Isolation and evaluation of proteolytic actinomycete isolates as novel inducers of pearl millet downy mildew disease protection. Sci Rep. 2016; 6:30789. PMC: 4976326. DOI: 10.1038/srep30789. View

14.

Bai Y, Cotrufo M . Grassland soil carbon sequestration: Current understanding, challenges, and solutions. Science. 2022; 377(6606):603-608. DOI: 10.1126/science.abo2380. View

15.

Chimwamurombe P, Gronemeyer J, Reinhold-Hurek B . Isolation and characterization of culturable seed-associated bacterial endophytes from gnotobiotically grown Marama bean seedlings. FEMS Microbiol Ecol. 2016; 92(6):fiw083. DOI: 10.1093/femsec/fiw083. View

16.

Bi S, Sourjik V . Stimulus sensing and signal processing in bacterial chemotaxis. Curr Opin Microbiol. 2018; 45:22-29. DOI: 10.1016/j.mib.2018.02.002. View

17.

Khameneh B, Iranshahy M, Soheili V, Bazzaz B . Review on plant antimicrobials: a mechanistic viewpoint. Antimicrob Resist Infect Control. 2019; 8:118. PMC: 6636059. DOI: 10.1186/s13756-019-0559-6. View

18.

Pan Y, Breidt Jr F, Kathariou S . Resistance of Listeria monocytogenes biofilms to sanitizing agents in a simulated food processing environment. Appl Environ Microbiol. 2006; 72(12):7711-7. PMC: 1694257. DOI: 10.1128/AEM.01065-06. View

19.

Koskella B, Taylor T, Bates J, Buckling A . Using experimental evolution to explore natural patterns between bacterial motility and resistance to bacteriophages. ISME J. 2011; 5(11):1809-17. PMC: 3197160. DOI: 10.1038/ismej.2011.47. View

20.

Normander B, Prosser J . Bacterial origin and community composition in the barley phytosphere as a function of habitat and presowing conditions. Appl Environ Microbiol. 2000; 66(10):4372-7. PMC: 92311. DOI: 10.1128/AEM.66.10.4372-4377.2000. View