Larvicidal and Repellent Effects of Essential Oils on the Brown Dog Tick (Rhipicephalus Sanguineus Sensu Lato) with Description of New Larval Repellent Activity Test Method

Overview

Journal Exp Appl Acarol

Specialties Biology
Parasitology

Date 2024 Feb 13

PMID 38351234

Authors

Samed Koc

Zeynep Nur Gultekin

Sevval Kahraman

Aysegul Cengiz

Burak Polat

Cansu Caliskan

Ozge Tufan-Cetin

Huseyin Cetin

Affiliations

Soon will be listed here.

Abstract

The aim of this research was to investigate the larvicidal and repellent effects of essential oils (EOs) obtained from two Lamiaceae plant species, Origanum minutiflorum O. Schwarz & P.H. Davis and Dorystoechas hastata Boiss. & Heldr. ex Bentham, both endemic to Turkey, on Rhipicephalus sanguineus s.l. Latreille (Acari: Ixodidae). The study also introduces a new test method that can be used to assess the repellent effects against ticks. Both plant EOs exhibited the highest larvicidal activity against brown dog tick larvae after 24 h and LC and LC values were determined as 0.101% and 0.125% for O. minutiflorum essential oil and 0.937% and 2.1% for D. hastata essential oil, respectively. In this study, we have described a detailed protocol for a novel larval repellent activity test (LRAT) for essential oils and extracts, using simple equipment. The advantages and limitations of LRAT, when compared to other tests commonly used to determine repellent effect against ticks, are also included in this study. The LRAT was developed with modifications of the larval immersion test (LIT) and proves to be a highly efficient and easily observable method. It can be used to test any active substance that may be toxic to humans and animals. According to the LRAT, at the end of 3 h, O. minutiflorum essential oil showed a high repellent effect, varying between 84.14% and 100% at 1% concentration. This result was not statistically different from the DEET, the positive control. When comparing the larvicidal and repellent activities, O. minutiflorum essential oil was found to be more effective than D. hastata essential oil.

Citing Articles

Exploring the Larvicidal and Repellent Potential of Taurus Cedar () Tar against the Brown Dog Tick ( sensu lato).

Koc S, Gultekin Z, Kahraman S, Cengiz A, Polat B, Caliskan C Molecules. 2023; 28(23).

PMID: 38067421 PMC: 10707419. DOI: 10.3390/molecules28237689.

References

Jongejan F, Uilenberg G . The global importance of ticks. Parasitology. 2005; 129 Suppl:S3-14. DOI: 10.1017/s0031182004005967. View

Cetin- H, Yanikoglu A . A study of the larvicidal activity of Origanum (Labiatae) species from southwest Turkey. J Vector Ecol. 2006; 31(1):118-22. DOI: 10.3376/1081-1710(2006)31[118:asotla]2.0.co;2. View

Eiden A, Kaufman P, Oi F, Allan S, Miller R . Detection of Permethrin Resistance and Fipronil Tolerance in Rhipicephalus sanguineus (Acari: Ixodidae) in the United States. J Med Entomol. 2015; 52(3):429-36. DOI: 10.1093/jme/tjv005. View

Sakkas H, Papadopoulou C . Antimicrobial Activity of Basil, Oregano, and Thyme Essential Oils. J Microbiol Biotechnol. 2016; 27(3):429-438. DOI: 10.4014/jmb.1608.08024. View

Daniele M, Dade M, Alvarez J, Reynaldi F, Errecalde J, Rodriguez-Vivas R . Current status of resistance to ivermectin in Rhipicephalus sanguineus sensu stricto infesting dogs in three provinces in Argentina. Vet Parasitol Reg Stud Reports. 2021; 26:100624. DOI: 10.1016/j.vprsr.2021.100624. View

Rodriguez-Vivas R, Ojeda-Chi M, Trinidad-Martinez I, Perez de Leon A . First documentation of ivermectin resistance in Rhipicephalus sanguineus sensu lato (Acari: Ixodidae). Vet Parasitol. 2017; 233:9-13. DOI: 10.1016/j.vetpar.2016.11.015. View

Koc S, Aydin L, Cetin H . Tick species (Acari: Ixodida) in Antalya City, Turkey: species diversity and seasonal activity. Parasitol Res. 2015; 114(7):2581-6. DOI: 10.1007/s00436-015-4462-7. View

Daemon E, Maturano R, Monteiro C, Goldner M, Massoni T . Acaricidal activity of hydroethanolic formulations of thymol against Rhipicephalus sanguineus (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Vet Parasitol. 2011; 186(3-4):542-5. DOI: 10.1016/j.vetpar.2011.11.013. View

Qiao Y, Yu Z, Bai L, Li H, Zhang S, Liu J . Chemical composition of essential oils from Thymus mongolicus, Cinnamomum verum, and Origanum vulgare and their acaricidal effects on Haemaphysalis longicornis (Acari: Ixodidae). Ecotoxicol Environ Saf. 2021; 224:112672. DOI: 10.1016/j.ecoenv.2021.112672. View

10.

Tabari M, Youssefi M, Barimani A, Araghi A . Carvacrol as a potent natural acaricide against Dermanyssus gallinae. Parasitol Res. 2015; 114(10):3801-6. DOI: 10.1007/s00436-015-4610-0. View

11.

Cetin H, Cilek J, Aydin L, Yanikoglu A . Acaricidal effects of the essential oil of Origanum minutiflorum (Lamiaceae) against Rhipicephalus turanicus (Acari: Ixodidae). Vet Parasitol. 2008; 160(3-4):359-61. DOI: 10.1016/j.vetpar.2008.11.009. View

12.

Gray J, Dantas-Torres F, Estrada-Pena A, Levin M . Systematics and ecology of the brown dog tick, Rhipicephalus sanguineus. Ticks Tick Borne Dis. 2013; 4(3):171-80. DOI: 10.1016/j.ttbdis.2012.12.003. View

13.

Benelli G, Pavela R . Repellence of essential oils and selected compounds against ticks-A systematic review. Acta Trop. 2017; 179:47-54. DOI: 10.1016/j.actatropica.2017.12.025. View

14.

Xie Y, Huang Q, Rao Y, Hong L, Zhang D . Efficacy of Origanum vulgare essential oil and carvacrol against the housefly, Musca domestica L. (Diptera: Muscidae). Environ Sci Pollut Res Int. 2019; 26(23):23824-23831. DOI: 10.1007/s11356-019-05671-4. View

15.

Salman M, Abbas R, Israr M, Abbas A, Mehmood K, Khan M . Repellent and acaricidal activity of essential oils and their components against Rhipicephalus ticks in cattle. Vet Parasitol. 2020; 283:109178. DOI: 10.1016/j.vetpar.2020.109178. View

16.

Cavanagh H, Wilkinson J . Biological activities of lavender essential oil. Phytother Res. 2002; 16(4):301-8. DOI: 10.1002/ptr.1103. View

17.

Becker S, Webster A, Doyle R, Martins J, Reck J, Klafke G . Resistance to deltamethrin, fipronil and ivermectin in the brown dog tick, Rhipicephalus sanguineus sensu stricto, Latreille (Acari: Ixodidae). Ticks Tick Borne Dis. 2019; 10(5):1046-1050. DOI: 10.1016/j.ttbdis.2019.05.015. View

18.

Selles S, Kouidri M, Gonzalez M, Gonzalez J, Sanchez M, Gonzalez-Coloma A . Acaricidal and Repellent Effects of Essential Oils against Ticks: A Review. Pathogens. 2021; 10(11). PMC: 8617816. DOI: 10.3390/pathogens10111379. View

19.

Isman M . Bioinsecticides based on plant essential oils: a short overview. Z Naturforsch C J Biosci. 2020; 75(7-8):179-182. DOI: 10.1515/znc-2020-0038. View

20.

Coskun S, Girisgin O, Kurkcuoglu M, Malyer H, Onur Girisgin A, Kirimer N . Acaricidal efficacy of Origanum onites L. essential oil against Rhipicephalus turanicus (Ixodidae). Parasitol Res. 2008; 103(2):259-61. DOI: 10.1007/s00436-008-0956-x. View