Multimodal Evaluation of the Effects of Low-intensity Ultrasound on Cerebral Blood Flow After Traumatic Brain Injury in Mice

Overview

Journal BMC Neurosci

Publisher Biomed Central

Specialty Neurology

Date 2024 Feb 13

PMID 38350864

Authors

Huiling Yi

Shuo Wu

Xiaohan Wang

Lanxiang Liu

Wenzhu Wang

Yan Yu

Zihan Li

Yinglan Jin

Jian Liu

Tao Zheng

Dan Du

Affiliations

Soon will be listed here.

Abstract

Traumatic brain injury (TBI) is one of the leading causes of death and disability worldwide, and destruction of the cerebrovascular system is a major factor in the cascade of secondary injuries caused by TBI. Laser speckle imaging (LSCI)has high sensitivity in detecting cerebral blood flow. LSCI can visually show that transcranial focused ultrasound stimulation (tFUS) treatment stimulates angiogenesis and increases blood flow. To study the effect of tFUS on promoting angiogenesis in Controlled Cortical impact (CCI) model. tFUS was administered daily for 10 min and for 14 consecutive days after TBI. Cerebral blood flow was measured by LSCI at 1, 3, 7 and 14 days after trauma. Functional outcomes were assessed using LSCI and neurological severity score (NSS). After the last test, Nissl staining and vascular endothelial growth factor (VEGF) were used to assess neuropathology. TBI can cause the destruction of cerebrovascular system. Blood flow was significantly increased in TBI treated with tFUS. LSCI, behavioral and histological findings suggest that tFUS treatment can promote angiogenesis after TBI.

References

Mazzeo A, Kunene N, Choi S, Gilman C, Bullock R . Quantitation of ischemic events after severe traumatic brain injury in humans: a simple scoring system. J Neurosurg Anesthesiol. 2006; 18(3):170-8. DOI: 10.1097/01.ana.0000210999.18033.f6. View

Alizada M, Lin S, Gao H . Recent advances in the treatment of traumatic brain injury with autologous and non-autologous multipotent stem and progenitor cells: preclinical models and clinical trials. Folia Neuropathol. 2021; 59(3):298-316. DOI: 10.5114/fn.2021.108536. View

Joseph B, Haider A, Rhee P . Traumatic brain injury advancements. Curr Opin Crit Care. 2015; 21(6):506-11. DOI: 10.1097/MCC.0000000000000247. View

Kraeuter A, Guest P, Sarnyai Z . The Y-Maze for Assessment of Spatial Working and Reference Memory in Mice. Methods Mol Biol. 2018; 1916:105-111. DOI: 10.1007/978-1-4939-8994-2_10. View

Wu S, Zheng T, Du J, Yuan Y, Shi Q, Wang Z . Neuroprotective effect of low-intensity transcranial ultrasound stimulation in endothelin-1-induced middle cerebral artery occlusion in rats. Brain Res Bull. 2020; 161:127-135. DOI: 10.1016/j.brainresbull.2020.05.006. View

Zheng T, Du J, Yuan Y, Wu S, Jin Y, Shi Q . Effect of Low Intensity Transcranial Ultrasound (LITUS) on Post-traumatic Brain Edema in Rats: Evaluation by Isotropic 3-Dimensional T2 and Multi-TE T2 Weighted MRI. Front Neurol. 2020; 11:578638. PMC: 7689022. DOI: 10.3389/fneur.2020.578638. View

Lv Y, Zhao P, Chen G, Sha Y, Yang L . Effects of low-intensity pulsed ultrasound on cell viability, proliferation and neural differentiation of induced pluripotent stem cells-derived neural crest stem cells. Biotechnol Lett. 2013; 35(12):2201-12. DOI: 10.1007/s10529-013-1313-4. View

Chen T, Lan T, Yang F . Low-Intensity Pulsed Ultrasound Attenuates LPS-Induced Neuroinflammation and Memory Impairment by Modulation of TLR4/NF-κB Signaling and CREB/BDNF Expression. Cereb Cortex. 2019; 29(4):1430-1438. DOI: 10.1093/cercor/bhy039. View

Oleary R, Nichol A . Pathophysiology of severe traumatic brain injury. J Neurosurg Sci. 2018; 62(5):542-548. DOI: 10.23736/S0390-5616.18.04501-0. View

10.

Kim J, Lee Y, Lee S, Kim K, Song M, Lee J . Mesenchymal Stem Cell Therapy and Alzheimer's Disease: Current Status and Future Perspectives. J Alzheimers Dis. 2020; 77(1):1-14. DOI: 10.3233/JAD-200219. View

11.

Su W, Wu C, Chen S, Yang F . Transcranial ultrasound stimulation promotes brain-derived neurotrophic factor and reduces apoptosis in a mouse model of traumatic brain injury. Brain Stimul. 2017; 10(6):1032-1041. DOI: 10.1016/j.brs.2017.09.003. View

12.

He R, Zhou W, Zhang Y, Hu S, Yu H, Luo Y . Combination of low-intensity pulsed ultrasound and C3H10T1/2 cells promotes bone-defect healing. Int Orthop. 2015; 39(11):2181-9. DOI: 10.1007/s00264-015-2898-0. View

13.

Mozaffari K, Dejam D, Duong C, Ding K, French A, Ng E . Systematic Review of Serum Biomarkers in Traumatic Brain Injury. Cureus. 2021; 13(8):e17056. PMC: 8428323. DOI: 10.7759/cureus.17056. View

14.

Yin J, Zhang H, Chen H, Lv Q, Jin X . Hypertonic Saline Alleviates Brain Edema After Traumatic Brain Injury via Downregulation of Aquaporin 4 in Rats. Med Sci Monit. 2018; 24:1863-1870. PMC: 5890824. DOI: 10.12659/msm.907212. View

15.

Eguchi K, Shindo T, Ito K, Ogata T, Kurosawa R, Kagaya Y . Whole-brain low-intensity pulsed ultrasound therapy markedly improves cognitive dysfunctions in mouse models of dementia - Crucial roles of endothelial nitric oxide synthase. Brain Stimul. 2018; 11(5):959-973. DOI: 10.1016/j.brs.2018.05.012. View

16.

Chen J, Li Y, Wang L, Zhang Z, Lu D, Lu M . Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. Stroke. 2001; 32(4):1005-11. DOI: 10.1161/01.str.32.4.1005. View

17.

Monti M, Schnakers C, Korb A, Bystritsky A, Vespa P . Non-Invasive Ultrasonic Thalamic Stimulation in Disorders of Consciousness after Severe Brain Injury: A First-in-Man Report. Brain Stimul. 2016; 9(6):940-941. DOI: 10.1016/j.brs.2016.07.008. View

18.

Dinda B, Dinda M, Kulsi G, Chakraborty A, Dinda S . Therapeutic potentials of plant iridoids in Alzheimer's and Parkinson's diseases: A review. Eur J Med Chem. 2019; 169:185-199. DOI: 10.1016/j.ejmech.2019.03.009. View

19.

Wu A, Ying Z, Gomez-Pinilla F . The salutary effects of DHA dietary supplementation on cognition, neuroplasticity, and membrane homeostasis after brain trauma. J Neurotrauma. 2011; 28(10):2113-22. PMC: 3191367. DOI: 10.1089/neu.2011.1872. View

20.

Siebold L, Obenaus A, Goyal R . Criteria to define mild, moderate, and severe traumatic brain injury in the mouse controlled cortical impact model. Exp Neurol. 2018; 310:48-57. DOI: 10.1016/j.expneurol.2018.07.004. View