Prokaryotic Expression, Purification, and the in Vitro and in Vivo Protection Study of Dehydrin WDHN2 from Triticum Aestivum

Overview

Journal Protoplasma

Publisher Springer

Specialty Biology

Date 2024 Feb 11

PMID 38342804

Authors

Hongmei Zhang

Jiafa Wu

Dandan Fu

Min Zhang

Lunji Wang

Minggui Gong

Affiliations

Soon will be listed here.

Abstract

Dehydrins proteins accumulate and play important protective roles in most plants during abiotic stresses. The objective of this study was to characterize a YSK-type dehydrin gene, WDHN2, isolated from Triticum aestivum previously. In this work, wheat dehydrin WDHN2 was expressed in Escherichia coli and purified by immobilized metal affinity chromatography, which exhibited as a single band by sodium dodecyl sulfonate polyacrylamide gel electrophoresis and western blotting. We show that WDHN2 is capable of alleviating lactate dehydrogenase inactivation from heat and desiccation in vitro enzyme activity protection assay. In vivo assay of Escherichia coli viability demonstrates the enhancement of cell survival by the overexpression of WDHN2. The protein aggregation prevention assay explores that WDHN2 has a broad protective effect on the cellular proteome. The results show that WDHN2 is mainly accumulated in the nucleus and cytosol, suggesting that this dehydrin may exert its function in both cellular compartments. Our data suggest that WDHN2 acts as a chaperone molecular in vivo.

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