Luminescent Bacteria As Bioindicators in Screening and Selection of Enzymes Detoxifying Various Mycotoxins

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2024 Feb 10

PMID 38339480

Authors

Elena Efremenko

Ilya Lyagin

Nikolay Stepanov

Olga Senko

Olga Maslova

Aysel Aslanli

Natalia Ugarova

Affiliations

Soon will be listed here.

Abstract

Interest in enzymes capable of neutralizing various mycotoxins is quite high. The methods used for the screening and selection of enzymes that catalyze the detoxification of mycotoxins should be sensitive and fast. However toxic compounds can be generated under the action of such enzymes. Thus, the assessment of the overall reduction in the toxic properties of reaction media towards bioluminescent bacteria seems to be the most reasonable control method allowing a quick search for the effective enzymatic biocatalysts. The influence of a wide range of mycotoxins and glucanases, which hydrolyze toxins with different chemical structures, on the analytical characteristics of luminescent photobacteria as a biosensing element has been studied. Different glucanases (β-glucosidase and endoglucanase) were initially selected for reactions with 10 mycotoxins based on the results of molecular docking which was performed in silico with 20 mycotoxins. Finally, the biorecognizing luminescent cells were used to estimate the residual toxicity of reaction media with mycotoxins after their interaction with enzymes. The notable non-catalytic decrease in toxicity of media containing deoxynivalenol was revealed with luminous cells for both types of tested glucanases, whereas β-glucosidase provided a significant catalytic detoxification of media with aflatoxin B and zearalenone at pH 6.0.

References

Hao W, Gu X, Yu X, Zhao Y, Li C, Jia M . Laccase Lac-W detoxifies aflatoxin B and degrades five other major mycotoxins in the absence of redox mediators. Environ Pollut. 2023; 338:122581. DOI: 10.1016/j.envpol.2023.122581. View

Li M, Wang Y, Ma L, Yan X, Lei Q . Dose-effect and structure-activity relationships of haloquinoline toxicity towards Vibrio fischeri. Environ Sci Pollut Res Int. 2021; 29(7):10858-10864. DOI: 10.1007/s11356-021-16388-8. View

Efremenko E, Maslova O, Stepanov N, Ismailov A . Using Cholinesterases and Immobilized Luminescent Photobacteria for the Express-Analysis of Mycotoxins and Estimating the Efficiency of Their Enzymatic Hydrolysis. Toxins (Basel). 2021; 13(1). PMC: 7825120. DOI: 10.3390/toxins13010034. View

Zaccaria M, Dawson W, Russel Kish D, Reverberi M, Bonaccorsi di Patti M, Domin M . Experimental-theoretical study of laccase as a detoxifier of aflatoxins. Sci Rep. 2023; 13(1):860. PMC: 9845376. DOI: 10.1038/s41598-023-27519-1. View

Trott O, Olson A . AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem. 2009; 31(2):455-61. PMC: 3041641. DOI: 10.1002/jcc.21334. View

Baker N, Sept D, Joseph S, Holst M, McCammon J . Electrostatics of nanosystems: application to microtubules and the ribosome. Proc Natl Acad Sci U S A. 2001; 98(18):10037-41. PMC: 56910. DOI: 10.1073/pnas.181342398. View

Singh G, Verma A, Kumar V . Catalytic properties, functional attributes and industrial applications of β-glucosidases. 3 Biotech. 2017; 6(1):3. PMC: 4697909. DOI: 10.1007/s13205-015-0328-z. View

Lyagin I, Efremenko E . Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action. Molecules. 2019; 24(13). PMC: 6651818. DOI: 10.3390/molecules24132362. View

Lyagin I, Maslova O, Stepanov N, Efremenko E . Degradation of mycotoxins in mixtures by combined proteinous nanobiocatalysts: In silico, in vitro and in vivo. Int J Biol Macromol. 2022; 218:866-877. DOI: 10.1016/j.ijbiomac.2022.07.179. View

10.

Sandlin N, Russell Kish D, Kim J, Zaccaria M, Momeni B . Current and Emerging Tools of Computational Biology To Improve the Detoxification of Mycotoxins. Appl Environ Microbiol. 2021; 88(3):e0210221. PMC: 8824263. DOI: 10.1128/AEM.02102-21. View

11.

Liu L, Xie M, Wei D . Biological Detoxification of Mycotoxins: Current Status and Future Advances. Int J Mol Sci. 2022; 23(3). PMC: 8835436. DOI: 10.3390/ijms23031064. View

12.

Dolinsky T, Czodrowski P, Li H, Nielsen J, Jensen J, Klebe G . PDB2PQR: expanding and upgrading automated preparation of biomolecular structures for molecular simulations. Nucleic Acids Res. 2007; 35(Web Server issue):W522-5. PMC: 1933214. DOI: 10.1093/nar/gkm276. View

13.

Awuchi C, Ondari E, Ogbonna C, Upadhyay A, Baran K, Okpala C . Mycotoxins Affecting Animals, Foods, Humans, and Plants: Types, Occurrence, Toxicities, Action Mechanisms, Prevention, and Detoxification Strategies-A Revisit. Foods. 2021; 10(6). PMC: 8228748. DOI: 10.3390/foods10061279. View

14.

Morris G, Huey R, Lindstrom W, Sanner M, Belew R, Goodsell D . AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility. J Comput Chem. 2009; 30(16):2785-91. PMC: 2760638. DOI: 10.1002/jcc.21256. View

15.

Pauliukaite R, Voitechovic E . Multisensor Systems and Arrays for Medical Applications Employing Naturally-Occurring Compounds and Materials. Sensors (Basel). 2020; 20(12). PMC: 7349305. DOI: 10.3390/s20123551. View

16.

Nesic K, Habschied K, Mastanjevic K . Possibilities for the Biological Control of Mycotoxins in Food and Feed. Toxins (Basel). 2021; 13(3). PMC: 8001018. DOI: 10.3390/toxins13030198. View

17.

Janik E, Niemcewicz M, Ceremuga M, Stela M, Saluk-Bijak J, Siadkowski A . Molecular Aspects of Mycotoxins-A Serious Problem for Human Health. Int J Mol Sci. 2020; 21(21). PMC: 7662353. DOI: 10.3390/ijms21218187. View

18.

Jian Q, Gong L, Li T, Wang Y, Wu Y, Chen F . Rapid Assessment of the Toxicity of Fungal Compounds Using Luminescent Vibrio qinghaiensis sp. Q67. Toxins (Basel). 2017; 9(10). PMC: 5666381. DOI: 10.3390/toxins9100335. View

19.

Yang J, Zhang Y . I-TASSER server: new development for protein structure and function predictions. Nucleic Acids Res. 2015; 43(W1):W174-81. PMC: 4489253. DOI: 10.1093/nar/gkv342. View

20.

Fisher M, Gurr S, Cuomo C, Blehert D, Jin H, Stukenbrock E . Threats Posed by the Fungal Kingdom to Humans, Wildlife, and Agriculture. mBio. 2020; 11(3). PMC: 7403777. DOI: 10.1128/mBio.00449-20. View