Effect of Carbohydrates on the Adhesion of Bordetella Bronchiseptica to the Respiratory Epithelium in Rabbits

Overview

Journal Vet Res Commun

Publisher Springer

Specialty Veterinary Medicine

Date 2024 Feb 9

PMID 38336962

Authors

Pilar Patino

Carolina Gallego

Nhora Martinez

Carlos Iregui

Alba Rey

Affiliations

Soon will be listed here.

Abstract

This study proposes an ecological approach for preventing respiratory tract infections caused by Bordetella bronchiseptica in mammals using a mixture of carbohydrates. In an in vivo study, 51-day-old New Zealand rabbits were treated with a solution containing 1 × 10 CFUs of B. bronchiseptica and 250 μg of one of the following carbohydrates: N acetylglucosamine (GlcNAc), N acetylgalactosamine (GalNAc), alpha methyl mannose (AmeMan), alpha methyl glucose (AmeGlc) and sialic acid (Neu5AC). Positive (B. bronchiseptica) and negative (Physiological Saline Solution (PSS)) controls were included. Animals treated with GlcNAc or AmeGlc showed no clinical signs of infection and exhibited a significant reduction (p < 0.05) in the severity of microscopic lesions evaluated in the nasal cavity and lung compared with the positive controls. Additionally, the presence of bacteria was not detected through microbiological isolation or PCR in the lungs of animals treated with these sugars. Use of a mixture of GlcNAc and AmeGlc resulted in greater inhibition of microscopic lesions, with a significant reduction (p < 0.05) in the severity of these lesions compared to the results obtained using individual sugars. Furthermore, the bacterium was not detected through microbiological isolation, Polymerase Chain Reaction (PCR) or indirect immunoperoxidase (IIP) in this group.

References

Fastres A, Canonne M, Taminiau B, Billen F, Garigliany M, Daube G . Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction. Vet Res. 2020; 51(1):46. PMC: 7092585. DOI: 10.1186/s13567-020-00769-x. View

Tuomanen E, Towbin H, Rosenfelder G, Braun D, Larson G, Hansson G . Receptor analogs and monoclonal antibodies that inhibit adherence of Bordetella pertussis to human ciliated respiratory epithelial cells. J Exp Med. 1988; 168(1):267-77. PMC: 2188977. DOI: 10.1084/jem.168.1.267. View

Chang P, Wang C, You C, Kao M . Effects of a HP0859 (rfaD) knockout mutation on lipopolysaccharide structure of Helicobacter pylori 26695 and the bacterial adhesion on AGS cells. Biochem Biophys Res Commun. 2011; 405(3):497-502. DOI: 10.1016/j.bbrc.2011.01.060. View

Zhao Z, Xue Y, Wang C, Ding K, Wu B, He Q . Antimicrobial susceptibility of Bordetella bronchiseptica isolates from pigs with respiratory diseases on farms in China. J Vet Med Sci. 2010; 73(1):103-6. DOI: 10.1292/jvms.10-0184. View

Davies J, Davies D . Origins and evolution of antibiotic resistance. Microbiol Mol Biol Rev. 2010; 74(3):417-33. PMC: 2937522. DOI: 10.1128/MMBR.00016-10. View

Sisti F, Fernandez J, Rodriguez M, Lagares A, Guiso N, Hozbor D . In vitro and in vivo characterization of a Bordetella bronchiseptica mutant strain with a deep rough lipopolysaccharide structure. Infect Immun. 2002; 70(4):1791-8. PMC: 127836. DOI: 10.1128/IAI.70.4.1791-1798.2002. View

Gallego C, Middleton A, Martinez N, Romero S, Iregui C . Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium. Vet Med Int. 2013; 2013:347086. PMC: 3608130. DOI: 10.1155/2013/347086. View

Edwards J, Groathouse N, Boitano S . Bordetella bronchiseptica adherence to cilia is mediated by multiple adhesin factors and blocked by surfactant protein A. Infect Immun. 2005; 73(6):3618-26. PMC: 1111863. DOI: 10.1128/IAI.73.6.3618-3626.2005. View

Muramatsu T . Protein-bound carbohydrates on cell-surface as targets of recognition: an odyssey in understanding them. Glycoconj J. 2001; 17(7-9):577-95. DOI: 10.1023/a:1011078627247. View

10.

Foley J, Rand C, Bannasch M, Norris C, Milan J . Molecular epidemiology of feline bordetellosis in two animal shelters in California, USA. Prev Vet Med. 2002; 54(2):141-56. DOI: 10.1016/s0167-5877(02)00022-3. View

11.

Ahmed M, Jahan R, Nissapatorn V, Wilairatana P, Rahmatullah M . Plant lectins as prospective antiviral biomolecules in the search for COVID-19 eradication strategies. Biomed Pharmacother. 2021; 146:112507. PMC: 8648558. DOI: 10.1016/j.biopha.2021.112507. View

12.

Rybolt L, Sabunwala S, Greene J . Zoonotic Bacterial Respiratory Infections Associated With Cats and Dogs: A Case Series and Literature Review. Cureus. 2022; 14(4):e24414. PMC: 9126301. DOI: 10.7759/cureus.24414. View

13.

Ellis J, Krakowka G, Dayton A, Konoby C . Comparative efficacy of an injectable vaccine and an intranasal vaccine in stimulating Bordetella bronchiseptica-reactive antibody responses in seropositive dogs. J Am Vet Med Assoc. 2003; 220(1):43-8. DOI: 10.2460/javma.2002.220.43. View

14.

Leininger E, Roberts M, Kenimer J, Charles I, Fairweather N, Novotny P . Pertactin, an Arg-Gly-Asp-containing Bordetella pertussis surface protein that promotes adherence of mammalian cells. Proc Natl Acad Sci U S A. 1991; 88(2):345-9. PMC: 50807. DOI: 10.1073/pnas.88.2.345. View

15.

Anderton T, Maskell D, Preston A . Ciliostasis is a key early event during colonization of canine tracheal tissue by Bordetella bronchiseptica. Microbiology (Reading). 2004; 150(Pt 9):2843-2855. DOI: 10.1099/mic.0.27283-0. View

16.

Savelkoul P, de Kerf D, Willems R, Mooi F, van der Zeijst B, Gaastra W . Characterization of the fim2 and fim3 fimbrial subunit genes of Bordetella bronchiseptica: roles of Fim2 and Fim3 fimbriae and flagella in adhesion. Infect Immun. 1996; 64(12):5098-105. PMC: 174494. DOI: 10.1128/iai.64.12.5098-5105.1996. View

17.

Sharon N . Carbohydrates as future anti-adhesion drugs for infectious diseases. Biochim Biophys Acta. 2006; 1760(4):527-37. DOI: 10.1016/j.bbagen.2005.12.008. View

18.

Idanpaan-Heikkila I, Simon P, Zopf D, Vullo T, Cahill P, SOKOL K . Oligosaccharides interfere with the establishment and progression of experimental pneumococcal pneumonia. J Infect Dis. 1997; 176(3):704-12. DOI: 10.1086/514094. View

19.

Mogensen T . Pathogen recognition and inflammatory signaling in innate immune defenses. Clin Microbiol Rev. 2009; 22(2):240-73, Table of Contents. PMC: 2668232. DOI: 10.1128/CMR.00046-08. View

20.

Dugal F, Girard C, Jacques M . Adherence of Bordetella bronchiseptica 276 to porcine trachea maintained in organ culture. Appl Environ Microbiol. 1990; 56(6):1523-9. PMC: 184465. DOI: 10.1128/aem.56.6.1523-1529.1990. View