Development of Novel SSR Markers and Validation by Assessing the Genetic Diversity of Endangered Deccan Mahseer, Tor Khudree

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2024 Feb 8

PMID 38329563

Authors

Gargee Das

Sofia Priyadarsani Das

Amrita Bit

Lakshman Sahoo

Subrat Kumar Swain

Channaveer Huchappa Raghavendra

N P Krishnaprasoon

Sangram Ketan Sahoo

Paramananda Das

Affiliations

Soon will be listed here.

Abstract

Background: The Deccan mahseer, Tor khudree (Sykes, 1839) is a potential game and food fish species belonging to the family cyprinidae and is categorized as endangered. Its distribution is restricted to southern part of India, specifically to Peninsular Rivers. This study is first to assess the genetic diversity and differentiation in Tor khudree by developing novel simple sequence repeat (SSR) markers.

Methods And Results: Low depth next generation sequencing followed by sequence analysis in MISA software identified 187,649 SSRs. The novel fourteen validated SSR loci were used for population genetic analysis. All of the SSR loci were highly informative with mean PIC > 0.5. High mean allelic richness (9.29) observed heterozygosity (0.98) and expected heterozygosity (0.79) were observed across the loci. However, genetic differentiation was low but significant (0.052). Negative F values were observed in both locus-wise and populations indicating the presence of high heterozygosity. Intrapopulation variation was found to be high (96.29%). The population structure revealed two genetic stocks.

Conclusions: The results from the present study including the highly polymorphic markers developed would be a useful resource for further research on population genetics and conservation genetics of the species.

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