NcRNAs-mediated Overexpression of Predicts Unfavorable Prognosis and Correlates with Immunotherapy Efficacy in Breast Cancer

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Feb 6

PMID 38318018

Authors

Yiyuan Liu

Jinyao Wu

Lingzhi Chen

Juan Zou

Qiuping Yang

Huiting Tian

Daitian Zheng

Zeqi Ji

Jiehui Cai

Zhiyang Li

Yexi Chen

Affiliations

Soon will be listed here.

Abstract

Breast cancer is the most frequent form of cancer in women and the primary cause of cancer-related deaths globally. DNA methylation and demethylation are important processes in human tumorigenesis. Ten-eleven translocation 3 () is a DNA demethylase. Prior research has demonstrated that is highly expressed in various human malignant tumors. However, the exact function and mechanism of in breast cancer remain unclear. In this study, we investigated expression in breast cancer and its correlation with clinicopathological characteristics of breast cancer patients. The results presented that expression was significantly increased in breast cancer and associated with the PAM50 subtype. Subsequently, we performed receiver operating characteristic, survival, and Cox hazard regression analyses. These results suggest that expression is associated with a poor prognosis and may be an indirect independent prognostic indicator in breast cancer. We also established a protein-protein interaction (PPI) network of and executed enrichment analyses of co-expressed genes, revealing their primary association with the cell cycle. Moreover, we identified noncoding RNAs (ncRNAs) contributing to overexpression using expression, correlation, and survival analyses. We identified the LINC01521/hsa-miR-29a-3p axis as the primary upstream ncRNA-related pathway in breast cancer. Furthermore, expression was positively associated with immune cell infiltration, immune cell biomarkers, and eight immune checkpoint gene expressions in breast cancer. expression also correlated with patient responses to immunotherapy. Finally, we conducted subcellular localization and immunohistochemical staining analysis of in breast cancer. We found that localized to the nucleoplasm, vesicles, and cytosol in the MCF-7 cell line, and expression was significantly upregulated in breast cancer tissues compared to para-tumor tissues. Our findings indicate that ncRNA-mediated overexpression of predicts an unfavorable prognosis and correlates with immunotherapy efficacy in breast cancer.

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