Resequencing and Characterization of the First Genome Isolated from Camel

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2024 Feb 5

PMID 38313017

Authors

Enrico Giovanelli Tacconi Gimenez

Marcus Vinicius Canario Viana

Thiago de Jesus Sousa

Flavia Aburjaile

Bertram Brenig

Artur Silva

Vasco Azevedo

Affiliations

Soon will be listed here.

Abstract

Background: is a zoonotic Gram-positive bacterial pathogen known to cause different diseases in many mammals, including lymph node abscesses in camels. Strains from biovars equi and ovis of can infect camels. Comparative genomics could help to identify features related to host adaptation, and currently strain Cp162 from biovar equi is the only one from camel with a sequenced genome.

Methods: In this work, we compared the quality of three genome assemblies of strain Cp162 that used data from the DNA sequencing platforms SOLiD v3 Plus, IonTorrent PGM, and Illumina HiSeq 2500 with an optical map and investigate the unique features of this strain. For this purpose, we applied comparative genomic analysis on the different Cp162 genome assembly versions and included other 129 genomes from the same species.

Results: Since the first version of the genome, there was an increase of 88 Kbp and 121 protein-coding sequences, a decrease of pseudogenes from 139 to 53, and two inversions and one rearrangement corrected. We identified 30 virulence genes, none associated to the camel host, and the genes and predicted to confer resistance to rifampin. In comparison to 129 genomes of the same species, strain Cp162 has four genes exclusively present, two of them code transposases and two truncated proteins, and the three exclusively absent genes , NUDIX domain protein, and Hypothetical protein. All 130 genomes had the rifampin resistance genes and . Our results found no unique gene that could be associated with tropism to camel host, and further studies should include more genomes and genome-wide association studies testing for genes and SNPs.

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