Exploration of Neuron Heterogeneity in Human Heart Failure with Dilated Cardiomyopathy Through Single-cell RNA Sequencing Analysis

Overview

Journal BMC Cardiovasc Disord

Publisher Biomed Central

Specialty Cardiology & Vascular Diseases

Date 2024 Feb 3

PMID 38310240

Authors

Yu-Hui Cui

Chun-Rong Wu

Dan Xu

Jian-Guo Tang

Affiliations

Soon will be listed here.

Abstract

Objective: We aimed to explore the heterogeneity of neurons in heart failure with dilated cardiomyopathy (DCM).

Methods: Single-cell RNA sequencing (scRNA-seq) data of patients with DCM and chronic heart failure and healthy samples from GSE183852 dataset were downloaded from NCBI Gene Expression Omnibus, in which neuron data were extracted for investigation. Cell clustering analysis, differential expression analysis, trajectory analysis, and cell communication analysis were performed, and highly expressed genes in neurons from patients were used to construct a protein-protein interaction (PPI) network and validated by GSE120895 dataset.

Results: Neurons were divided into six subclusters involved in various biological processes and each subcluster owned its specific cell communication pathways. Neurons were differentiated into two branches along the pseudotime, one of which was differentiated into mature neurons, whereas another tended to be involved in the immune and inflammation response. Genes exhibited branch-specific differential expression patterns. FLNA, ITGA6, ITGA1, and MDK interacted more with other gene-product proteins in the PPI network. The differential expression of FLNA between DCM and control was validated.

Conclusion: Neurons have significant heterogeneity in heart failure with DCM, and may be involved in the immune and inflammation response to heart failure.

Citing Articles

Combining Bulk and Single Cell RNA-Sequencing Data to Identify Hub Genes of Fibroblasts in Dilated Cardiomyopathy.

Huang X, Zhao X, Li Y, Feng Y, Zhang G, Wang Q J Inflamm Res. 2024; 17:5375-5388.

PMID: 39161677 PMC: 11330748. DOI: 10.2147/JIR.S470860.

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