Langerhans Cell Expression in Oral Submucous Fibrosis: An Immunohistochemical Analysis

Overview

Journal J Oral Maxillofac Pathol

Date 2024 Feb 2

PMID 38304517

Authors

Shugufta Shafi

Rubeena Anjum

Mandeep Kaur

Nidhi Khajuria

Affiliations

Soon will be listed here.

Abstract

Introduction: Langerhans cells (LCs) are dendritic cells (DCs) of the epithelium which play a role in an array of oral lesions from gingivitis to oral cancer. Oral submucous fibrosis (OSMF), a potentially malignant disorder (PMD), is an insidious chronic disease with juxta-epithelial inflammatory changes leading to fibrosis. LCs may play a part in the ongoing inflammatory dysregulation of OSMF.

Objective: The study was aimed at elucidating the distribution of LCs in varying grades of OSMF.

Materials And Methods: A retrospective study using 18 cases of OSMF, graded using haematoxylin and eosin (H&E)-stained section. Immunohistochemistry was performed using polyclonal anti-CD1a antibodies to identify LCs in six cases of normal tissue and 18 samples of OSMF. The distribution of LCs among the various grades and normal mucosa analysed using Student's t-test.

Results: LC population in the OSMF was significantly higher when compared to the normal epithelium (p < 0.001). Within the grades, the advanced stage had more LCs than the other stages.

Conclusion: The increase in LCs might indicate the role of antigenic exposure in turn leading to cell-mediated immunity in OSMF. Thus, the fibrosis in OSMF might have a direct link to LCs.

References

Banchereau J, Steinman R . Dendritic cells and the control of immunity. Nature. 1998; 392(6673):245-52. DOI: 10.1038/32588. View

Ferrisse T, de Oliveira A, Palacon M, da Silveira H, Massucato E, Yamamoto de Almeida L . Immunohistochemical evaluation of Langerhans cells in oral lichen planus and oral lichenoid lesions. Arch Oral Biol. 2021; 124:105027. DOI: 10.1016/j.archoralbio.2020.105027. View

Daniels T . Human mucosal Langerhans cells: postmortem identification of regional variations in oral mucosa. J Invest Dermatol. 1984; 82(1):21-4. DOI: 10.1111/1523-1747.ep12259038. View

Narayanan B, Narasimhan M . Langerhans Cell Expression in Oral Submucous Fibrosis: An Immunohistochemical Analysis. J Clin Diagn Res. 2015; 9(7):ZC39-41. PMC: 4573035. DOI: 10.7860/JCDR/2015/12608.6196. View

Tilakaratne W, Klinikowski M, Saku T, Peters T, Warnakulasuriya S . Oral submucous fibrosis: review on aetiology and pathogenesis. Oral Oncol. 2005; 42(6):561-8. DOI: 10.1016/j.oraloncology.2005.08.005. View

Haque M, Meghji S, Khitab U, Harris M . Oral submucous fibrosis patients have altered levels of cytokine production. J Oral Pathol Med. 2000; 29(3):123-8. DOI: 10.1034/j.1600-0714.2000.290304.x. View

Jaitley S, Saraswathi T . Pathophysiology of Langerhans cells. J Oral Maxillofac Pathol. 2012; 16(2):239-44. PMC: 3424941. DOI: 10.4103/0973-029X.99077. View

Cruchley A, Williams D, Farthing P, Lesch C, Squier C . Regional variation in Langerhans cell distribution and density in normal human oral mucosa determined using monoclonal antibodies against CD1, HLADR, HLADQ and HLADP. J Oral Pathol Med. 1989; 18(9):510-6. DOI: 10.1111/j.1600-0714.1989.tb01353.x. View

Steinman R, COHN Z . Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution. J Exp Med. 1973; 137(5):1142-62. PMC: 2139237. DOI: 10.1084/jem.137.5.1142. View

10.

Deckers J, Hammad H, Hoste E . Langerhans Cells: Sensing the Environment in Health and Disease. Front Immunol. 2018; 9:93. PMC: 5799717. DOI: 10.3389/fimmu.2018.00093. View

11.

Haque M, Harris M, Meghji S, Barrett A . Immunolocalization of cytokines and growth factors in oral submucous fibrosis. Cytokine. 1998; 10(9):713-9. DOI: 10.1006/cyto.1997.0342. View

12.

Lasisi T, Oluwasola A, Lasisi O, Akang E . Association between langerhans cells population and histological grade of oral squamous cell carcinoma. J Oral Maxillofac Pathol. 2014; 17(3):329-33. PMC: 3927330. DOI: 10.4103/0973-029X.125177. View

13.

Cutler C, Jotwani R . Antigen-presentation and the role of dendritic cells in periodontitis. Periodontol 2000. 2004; 35:135-57. DOI: 10.1111/j.0906-6713.2004.003560.x. View

14.

Barrett A, Cruchley A, Williams D . Oral mucosal Langerhans' cells. Crit Rev Oral Biol Med. 1996; 7(1):36-58. DOI: 10.1177/10454411960070010301. View

15.

Silva L, Fonseca F, Almeida O, Mariz B, Lopes M, Radhakrishnan R . CD1a+ and CD207+ cells are reduced in oral submucous fibrosis and oral squamous cell carcinoma. Med Oral Patol Oral Cir Bucal. 2019; 25(1):e49-e55. PMC: 6982989. DOI: 10.4317/medoral.23177. View

16.

PINDBORG J, SIRSAT S . Oral submucous fibrosis. Oral Surg Oral Med Oral Pathol. 1966; 22(6):764-79. DOI: 10.1016/0030-4220(66)90367-7. View

17.

Baer R, Thorbecke G . Langerhans cells: a review of their nature with emphasis on their immunologic functions. Prog Allergy. 1978; 24:268-94. View

18.

Matsue H, Cruz Jr P, Bergstresser P, Takashima A . Cytokine expression by epidermal cell subpopulations. J Invest Dermatol. 1992; 99(5):42S-45S. DOI: 10.1111/1523-1747.ep12668619. View

19.

Haque M, Harris M, Meghji S, Speight P . An immunohistochemical study of oral submucous fibrosis. J Oral Pathol Med. 1997; 26(2):75-82. DOI: 10.1111/j.1600-0714.1997.tb00025.x. View

20.

Xie Y, Zhang X, Inoue Y, Wakasugi S, Makino T, Ihn H . Expression of CD1a and CD86 on scleroderma Langerhans cells. Eur J Dermatol. 2007; 18(1):50-4. DOI: 10.1684/ejd.2008.0310. View