Lysine Demethylase 3A Promotes Chondrogenic Differentiation of Aged Human Dental Pulp Stem Cells

Overview

Journal J Dent Sci

Specialty Dentistry

Date 2024 Feb 2

PMID 38303882

Authors

Qiannan Sun

Rushui Bai

Si Chen

Zimeng Zhuang

Jie Deng

Tianyi Xin

Yunfan Zhang

Qian Li

Bing Han

Affiliations

Soon will be listed here.

Abstract

Background/purpose: Aging severely impairs the beneficial effects of human dental pulp stem cells (hDPSCs) on cartilage regeneration. Lysine demethylase 3A (KDM3A) is involved in regulating mesenchymal stem cells (MSCs) senescence and bone aging. In this study, we investigated the role of KDM3A in hDPSCs aging and whether KDM3A could rejuvenate aged hDPSCs to enhance their chondrogenic differentiation capacity.

Materials And Methods: The cellular aging of hDPSCs was evaluated by senescence-associated β-galactosidase (SA-β-gal) staining. Protein levels were determined using Western blot analysis. KDM3A was overexpressed in aged hDPSCs by lentivirus infection. Quantitative reverse-transcription polymerase chain reaction (RT-qPCR) were used to determine the mRNA levels of stemness markers. Toluidine blue staining was used to evaluate the effect of KDM3A overexpression on the chondrogenic differentiation of aged hDPSCs.

Results: hDPSCs at passage 12 or treated with etoposide exhibited augmented cellular senescence as evidenced by increased SA-β-gal activity. KDM3A was significantly increased during senescence of hDPSCs. Overexpression of KDM3A did not affect the stemness properties but significantly promoted the chondrogenic differentiation of aged hDPSCs.

Conclusion: Our findings indicate that KDM3A plays an important role in the maintenance of the chondrogenic differentiation capacity of aged hDPSCs and suggest that therapies targeting KDM3A may be a novel strategy to rejuvenate aged hDPSCs.

Citing Articles

Dynamic changes in chromatin accessibility during the differentiation of dental pulp stem cells reveal that induction of odontogenic gene expression is linked with specific enhancer construction.

Sasaki K, Suzuki S, Fahreza R, Nemoto E, Yamada S J Dent Sci. 2024; 19(3):1705-1713.

PMID: 39035322 PMC: 11259614. DOI: 10.1016/j.jds.2023.10.022.

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