Nudt21-mediated Alternative Polyadenylation of MZT1 3'UTR Contributes to Pancreatic Cancer Progression

Overview

Journal iScience

Publisher Cell Press

Date 2024 Feb 2

PMID 38303721

Authors

Yu Zhou

Jiabin Yang

Leyi Huang

Chao Liu

Min Yu

Rufu Chen

Quanbo Zhou

Affiliations

Soon will be listed here.

Abstract

Alternative polyadenylation (APA) is an important post-transcriptional regulatory mechanism and is involved in many diseases, but its function and mechanism in regulating pancreatic cancer (PC) pathogenesis remain unclear. In this study, we found that the 3' UTR shortening of MZT1 was the most prominent APA event in PC liver metastases. The short-3'UTR isoform exerted a stronger effect in promoting cell proliferation and migration both and . NUDT21, a core cleavage factor involved in APA, promoted the usage of proximal polyadenylation sites (PASs) on MZT1 mRNA by binding to the UGUA element located upstream of the proximal PAS. High percentage of distal polyA site usage index of MZT1 was significantly associated with a better prognosis. These findings demonstrate a crucial mechanism that NUDT21-mediated APA of MZT1 could promote the progression of PC. Our findings provided a better understanding of the connection between PC progression and APA machinery.

Citing Articles

Characterization and molecular targeting of CFIm25 (NUDT21/CPSF5) mRNA using miRNAs.

Khan N, Gupta M, Masamha C FASEB J. 2025; 39(2):e70324.

PMID: 39812508 PMC: 11760631. DOI: 10.1096/fj.202402184R.

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