Article: Isolation of metastatic B16 melanoma variants using anti-Met 72 monoclonal antibodies and flow cytometry

Analysis of a number of B16 melanoma clones has revealed a high correlation between metastatic activity and the quantitative expression of a 72,000 dalton glycoprotein, Met 72. In the present study, metastatic tumor cell variants have been directly isolated from a heterogeneous, poorly metastatic melanoma (B16-F1) by anti-Met 72 monclonal antibodies and cell sorting procedures. These studies provide direct proof that Met-72 antigens are in fact surface markers of B16 melanoma metastatic variants and may provide the means of monitoring their presence, influence and autonomy during tumor progression.

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References

1.

Nicolson G . Cancer metastasis. Organ colonization and the cell-surface properties of malignant cells. Biochim Biophys Acta. 1982; 695(2):113-76. DOI: 10.1016/0304-419x(82)90020-8. View

2.

Xiang J, Kimura A . In vitro modulation of the metastatic phenotype. I. Analysis of differentiation forms of the B16 melanoma expressing Met-72 determinants and metastatic activity. Clin Exp Metastasis. 1986; 4(4):293-309. DOI: 10.1007/BF00133594. View

3.

Kimura A, Xiang J . High levels of Met-72 antigen expression: correlation with metastatic activity of B16 melanoma tumor cell variants. J Natl Cancer Inst. 1986; 76(6):1247-54. View

4.

Foulds L . The histologic analysis of mammary tumors of mice. J Natl Cancer Inst. 1956; 17(6):701-801. View

5.

Kripke M, Gruys E, Fidler I . Metastatic heterogeneity of cells from an ultraviolet light-induced murine fibrosarcoma of recent origin. Cancer Res. 1978; 38(9):2962-7. View

6.

Fidler I, Gruys E, Cifone M, Barnes Z, Bucana C . Demonstration of multiple phenotypic diversity in a murine melanoma of recent origin. J Natl Cancer Inst. 1981; 67(4):947-56. View

7.

Harris J, Chambers A, Hill R, Ling V . Metastatic variants are generated spontaneously at a high rate in mouse KHT tumor. Proc Natl Acad Sci U S A. 1982; 79(18):5547-51. PMC: 346941. DOI: 10.1073/pnas.79.18.5547. View

8.

Hulett H, Bonner W, Sweet R, Herzenberg L . Development and application of a rapid cell sorter. Clin Chem. 1973; 19(8):813-6. View

9.

Olsson L, FORCHHAMMER J . Induction of the metastatic phenotype in a mouse tumor model by 5-azacytidine, and characterization of an antigen associated with metastatic activity. Proc Natl Acad Sci U S A. 1984; 81(11):3389-93. PMC: 345513. DOI: 10.1073/pnas.81.11.3389. View

10.

Poste G . Experimental systems for analysis of the malignant phenotype. Cancer Metastasis Rev. 1982; 1(2):141-99. DOI: 10.1007/BF00048224. View

11.

Nowell P . The clonal evolution of tumor cell populations. Science. 1976; 194(4260):23-8. DOI: 10.1126/science.959840. View

12.

Fidler I, Kripke M . Metastasis results from preexisting variant cells within a malignant tumor. Science. 1977; 197(4306):893-5. DOI: 10.1126/science.887927. View

Isolation of Metastatic B16 Melanoma Variants Using Anti-Met 72 Monoclonal Antibodies and Flow Cytometry