Isolation of Active Compounds from GMY01 and Cytotoxic Activity on Breast Cancer Cells Line

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Jan 31

PMID 38293453

Authors

Rifki Febriansah

Triana Hertiani

Jaka Widada

Muhammad Taher

Ema Damayanti

Mustofa Mustofa

Affiliations

Soon will be listed here.

Abstract

The occurrence of resistance to anticancer and the emergence of serious side effects due to chemotherapy is one of the main problems in cancer treatment, including breast cancer. The need for effective anticancer with a specific target is urgently required. are widely known as the potential producers of new anticancer molecules. Previously reported that the methanol extract of GMY01 isolated from Krakal Coast, Gunungkidul had very strong cytotoxic activity against MCF-7 and T47D breast cancer cells with IC values of 0.6 and 1.3 μg/mL, respectively. The following study aimed to isolate and identify active compounds of the GMY01 and evaluate its cytotoxic activity. The study was started by re-culturing and re-fermented optimization of GMY01 in a larger volume, then the bacteria were extracted using methanol following the bioassay-guided isolation of the extract obtained. The active compounds obtained were then structurally determined using UV/Vis spectroscopy, Fourier Transform-Infrared (FT-IR), Liquid Chromatography-Mass Spectroscopy (LC-MS), H NMR, and C NMR and analyzed for their cytotoxic activity using MTT assay on MCF-7 and normal Vero cells line. The results showed that the culture of the GMY01 using Starch Nitrate Broth (SNB) media yields the best results compared to other culture media. An active anticancer compound namely mannotriose was successfully isolated from the methanol extract with an IC value of 5.6 μg/mL and 687 μg/mL against the MCF-7 and Vero cells lines, respectively, indicating that this compound showed strong cytotoxic activity with high selectivity.

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