Phosphates As Assisting Groups in Glycan Synthesis

Overview

Journal ACS Cent Sci

Specialty Chemistry

Date 2024 Jan 31

PMID 38292611

Authors

Eric T Sletten

Giulio Fittolani

Nives Hribernik

Marlene C S Dal Colle

Peter H Seeberger

Martina Delbianco

Affiliations

Soon will be listed here.

Abstract

In nature, phosphates are added to and cleaved from molecules to direct biological pathways. The concept was adapted to overcome limitations in the chemical synthesis of complex oligosaccharides. Phosphates were chemically placed on synthetic glycans to ensure site-specific enzymatic elongation by sialylation. In addition, the deliberate placement of phosphates helped to solubilize and isolate aggregating glycans. Upon traceless removal of the phosphates by enzymatic treatment with alkaline phosphatase, the native glycan structure was revealed, and the assembly of glycan nanostructures was triggered.

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